DNA

Question 1

Step 1

Answer 1

• grind with a pestle and mortar
• breaks cells wall

Question 2

Step 2

Answer 2

• Mix in detergent
• break down cell membrane
• releases cell contents

Question 3

Step 3

Answer 3

• add salt
• breaks hydrogen bond between DNA molecules and water

Question 4

Step 4

Answer 4

• add a protease enzyme
• breaks down histones associated with DNA

Question 5

Step 5

Answer 5

• add ethanol
• cause DNA to precipitate out of the solution

Question 6

Pick up DNA

Answer 6

• should form a white strand layer between solution and alcohol layer
• can be spooned out by a glass rod