Diagnostic tools utilizing monoclonal antibodies

Question 1

Antibody/immunoglobulin

Answer 1

a protein produced by plasma cells that is used by the immune system to neutralize pathogens / foreign proteins.

Question 2

Monoclonal antibodies (mAb or moAb)

Answer 2

antibodies made by identical B cells that are all the same as a unique parent cell. They bind to the same epitope (the part of an antigen that is recognized by the antibody). Highly specific for the antigen

Question 3

ELISA

Answer 3

Enzyme-linked immunosorbent assay.

Question 4

Flow Cytometry

Answer 4

is the measurement of various cell properties (“cytometry”) as they move in single file (“flow”) in a fluid column and interrupt a beam of laser light.