3.2. Recombinant Proteins Flashcards
1
Q
Origin of replication
A
Site where DNA replication is initiated
2
Q
Marker genes for selection and/ screening
A
- Selectable marker
- I.e. antibiotic resistance
- allows cells to grow on media contains antibiotic
- only cells containing plasmid form colonies - Screenable marker for recombinant molecules
- allows screening of different phenotypes
3
Q
Unique RE sites
A
Allows inserts to be cloned in specific sites on the plasmid
4
Q
Promoters for gene expression
A
Allows expression of cloned gene in the vector
5
Q
rProtein
A
Recombinant proteins
6
Q
rDNA
A
Recombinant DNA
7
Q
RE sites
A
Restriction endonuclease sites
8
Q
Vectors
A
- Self-replicating DNA molecules used to transfer foreign DNA between host cells
- Ideal vector = small in size with a single restriction endonuclease site
9
Q
Plasmids
A
- Contained in bacteria, extrachromosomal molecules that are circular and double-stranded
10
Q
Bacteriophages
A
Viruses that infect bacteria
11
Q
Cosmids
A
Specialized plasmids with DNA sequences called Cos sites
12
Q
Inclusion bodies
A
Insoluble aggregates which occur when eukaryotic proteins do not fold properly in E. Coli
13
Q
CHO
A
Chinese hamster cells
