Devin • Baccillus

Question 1

Bacillus anthracis plasmid name?

Answer 1

plasmid pX01

Question 2

plasmid pX01 of Bacillus anthracis codes for?

Answer 2

protective agent (PA), edema factor (EF) and lethal factor (LF)
o	PA + EF = edema toxin
o	PA + LF = lethal toxin

Question 3

lethal factor (LF)

Answer 3

zing-dependent metalloprotease that cleaves MAP kinase, leading to inhibition of innate immunity, cell death, and release of inflammatory cytokines

Question 4

lethal factor (LF)

Answer 4

calmodulin-dependent adenylate cyclase that increases cAMP

o results in edema and inhibition of innate immunity

Question 5

Bacillus anthracis has a polypeptide capsule?

Answer 5

yes! polypeptide capsule – poly-D-glutamic acid

Question 6

Bacillus anthracis plasmid pX02 carries what info?

Answer 6

carries capA, capB, and capC that synthesize the polypeptide capsule

Question 7

Bacillus anthracis has a special stain for what?

Answer 7

spore stain (malachite green stain) used to visualize spores in specimens

Question 8

Bacillus cereus heat-stable, proteolysis-resistant enterotoxin =

Answer 8

emetic form of disease (rice)

Question 9

Bacillus cereus heat-labile enteroxin =

Answer 9

diarrheal form of disease

•induces adenylate cyclase activity

Question 10

Bacillus cereus ocular infections have 3 toxins?

Answer 10

• necrotic toxin – heat labile enterotoxin
• cereolysin – potent hemolysin named after species
• phospholipase C – potent lecithinase