Day 7(4): Visualizing the Ocular Fundus Flashcards

1
Q

What is direct ophthalmoscopy?

A

Visualizing the fundus using a direct ophthalmoscope

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2
Q

Indications for direct ophthalmoscopy.

A
  1. Need for a high power study of the optic disc, macula and SMALL lesions in the posterior pole
  2. Measure elevations and depressions:
    - slit beam curved back: depression
    - slit beam curved forward: elevation
  3. Measure dioptric power of the eye (myopia, hyperopia or emmetropia)
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3
Q

Advantages of direct ophthalmoscopy.

A
  1. High magnifications: 15X
  2. Erect image
  3. Ability to take measurements
  4. Easy to learn
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4
Q

Disadvantages of direct ophthalmoscopy.

A
  1. Limited field of view: 10 - 12 degrees
    - can only view upto the equator
    - due to small aperture
  2. Poor illumination: affected by media opacities
  3. Monocular: loss of binocularity and stereopsis
  4. Distortion in the periphery
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5
Q

What is the near point?

A
  • Closest point at which an object can be placed and still form a focused image ON the retina within the eye’s accommodative range
  • 8.0 inches or 20 cm: after adjusting for AL
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6
Q

Steps in doing direct ophthalmoscopy.

A

Set-up:
- Pt sitting upright
- Focused at an object at a distance
- Dim lights
- Pupils pharmacologically dilated

Examiner position:
- Scope resting against the cheek
- Standing or sitting obliquely to the pt’s side
- Do NOT obstruct pt’s fixation point
- Pt’s R eye = Examiner’s R eye = Ophthalmoscope in R hand
- Pt’s L eye = Examiner’s L eye = Ophthalmoscope in L hand

Steps:
1. Shine light from a short distance and slowly move closer.
2. Adjust light aperture:
- too much light: uncomfortable for pt
- too little light: poor illumination of internal eye
3. Look for Red-Orange Reflex: move towards that direction as close as possible, almost touching the eye with fingers touching the pt’s cheek
4. Look for the optic disc then adjust focus until clear.
5. Follow vessels emanating from the disc and examine each quadrant
6. Move temporally to examine macula/fovea last.
- will cause intense glare and discomfort as this is the most sensitive area to light

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7
Q

What is indirect ophthalmoscopy?

A

Visualizing the fundus using an indirect ophthalmoscope

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8
Q

Indications for indirect ophthalmoscopy.

A
  1. (+) media opacities: due to stronger illumination
  2. High refractive errors: less distortion
  3. Children
  4. Total fundus examination: upto periphery and pars plana
  5. Examination of LARGE lesions: due to wider field of view
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9
Q

Advantages of indirect ophthalmoscopy.

A
  1. Wider field of view: 30 - 35 degrees
    - vs 10 - 12 degrees in DO
    - peripheral retina (ora serrata) to pars plana can be examined
  2. Stronger illumination: can penetrate media opacities
  3. Stereopsis: due to binocularity
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10
Q

Disadvantages of indirect ophthalmoscopy.

A
  1. Low magnification: 2 - 5X
    - vs 15X in DO
  2. Inverted image
    - vs erect in DO
  3. Harder to master
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11
Q

Prerequisites for proper indirect ophthalmoscopy.

A
  1. Maximal mydriasis: wider field of view
  2. Good control of the eye: pt able to fixate, understand and cooperate; NO nystagmus
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12
Q

What is stereopsis?

A
  • Perception of depth and three-dimensionality
  • Requirement: binocularity or a pair of optimally functioning eyes
  • Result of retinal disparity: images formed on the left and right retina are different causing the visual cortex to integrate both images and perceive difference as depth
  • Objects should be at a DISTANCE: accommodation is relaxed
  • Problems with NEAR objects:
    1. If object is closer than the near point (8 inches), image will form in front of the examiner’s retina thus losing focus
    2. Fusional Vergence Amplitude: limited inward rotation of the eyes to maintain focus
    3. Amplitude of accommodation: limited accommodative power of the examiner’s lens
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13
Q

How does indirect ophthalmoscopy work to view the fundus stereoscopically?

A

To use binocular vision and stereopsis:
- need to move object FARTHER away to neutralize the near point, vergence and accommodation
- problems:
1. loss of focus: object may not fall at the line of sight
2. as you move farther away, the visualized area of the retina gets SMALLER: limited by pupillary aperture
3. difficulty perceiving minute details and pathologies

Solutions:
1. Reflecting mirrors: bend or focus the line of sight
2. Strong illumination
3. Condensing lens: to overcome the limited accommodation of examiner’s lens at close distances

Image: forms BETWEEN examiner and condensing lens
1. Real
2. Inverted

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14
Q

Steps in doing indirect ophthalmoscopy.

A

Set-up:
- Pt lying down
- Fixated at a distance
- Dim lights
- Pupils pharmacologically dilated

Examiner position:
- Standing above the pt’s head or at the side
- Arms stretched
- Headpiece, lens and examiner’s arms moving as a unit
- Thumb or middle finger to keep eye open
- Lens positioned one-finger length from the pt’s eye
- Lens held by both index fingers and thumbs with the other fingers resting on the periorbital area

Steps:
1. Adjust head piece to desired fit.
2. Adjust pupillary distance of eyepieces until SINGLE image is seen with good focus and depth
3. Adjust light source to a CENTRAL position.
4. Systematically examine the internal eye as in DO:
- optic disc
- retinal vessels: follow each in all 4 quadrants to the periphery
- peripheral retina and ora serrata
- ciliary body (pars plana)
- macula/fovea: examine last to avoid glare and discomfort as this is the most sensitive area to light

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15
Q

What are the common lenses used for IO?

A

20D Large
- Double aspheric lens
- Volk Optical
- M: 3.13X
- F: 46 degrees (static), 60 degrees (dynamic)

28D Large
- Volk Optical
- M: 2.27X
- F: 53 degrees (static), 68 degrees (dynamic)

Note: HIGHER dioptric power = WIDER field of view = SMALLER magnification
- D and F are DIRECTLY proportional: the higher the power, the wider the field illuminated
- F and M are INVERSELY proportional: the wider the field illuminated and examined, the smaller the image

Optional contraptions:
1. Adapter Set
- transforms standard 20D lens into 16D, 24D or 28D
2. Yellow Filter
- decreases light scatter and glare to increase comfort
- enhances image contrast

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16
Q

Discuss the parts of the Retinal or Amsler Dubois Chart.

A
  • Consisting of 7 concentric circles bisected by 12 radial lines representing clock hands
  • Divides retina into 2 zones:

Zone 1: Posterior Fundus (32 - 42 mm)
1. Foveola
- point in the center of the chart
2. Fovea
- 1.5 mm inner circle
- midway between line VI and XII
3. Macula
- 5.5 mm inner circle
4. Posterior Pole/Area Centralis
- 10 mm inner circle
- contains circle representing optic disc (1.5 mm) nasal to the fovea
- if optic disc found in the R of the VI and XII lines = R eye
5. Midperipheral Retina
- zone beyond posterior pole upto the posterior edge of the vortex vein ampulla

Junction: Imaginary circle connecting vortex veins AMPULLA
- outer border of the posterior fundus
- delineates the posterior fundus from the anterior/far peripheral retina

Zone 2: Peripheral Retina (9 mm area outer to posterior fundus)
5. Equator
- 3 mm outer to imaginary circle connecting vortex veins AMPULLA
- circle connecting bulk of vortex veins
6. Ora Serrata
- junction of the peripheral retina and ciliary body
- 6 mm outer to the equator
- temporal: smooth
- nasal: jagged
7. Junction of Pars Plana and Pars Plicata
- outermost circle
- Pars Plana: zone between 2nd and outermost circle
- Pars Plicata: area beyond the outermost circle

17
Q

Discuss proper Retinal Chart orientation.

A

Remember: IO image is real but INVERTED

Situation 1: Pt lying down with examiner positioned above the head
- inverted image neutralized by examiner’s inverted position: inverting the inverted image = upright
- what is seen is the UPRIGHT image: draw findings as you see it
+ Temporal image = Temporal retina
+ Nasal image = Nasal retina
+ Superior image = Superior retina
+ Inferior image = Inferior retina

Situation 2: Pt sitting down with examiner positioned in front
- what is seen is the INVERTED image
- draw everything in the opposite location or flip the drawing
+ Temporal image = Nasal retina
+ Nasal image = Temporal retina
+ Superior image = Inferior retina
+ Inferior image = Superior retina

Reminders:
1. Disregard orientation while drawing. Just draw as you see it. Then flip image if needed based on the examining position.
2. Peripheral or anterior structures appear closer in the condensing lens.
3. Ora serrata temporally is SMOOTH while nasally is JAGGED.

18
Q

Compare DO and IO.

A

Direct Ophthalmoscopy: magnified but limited field
Magnification: more magnified (15X)
Field of View: limited (10 - 12 degrees)
Illumination: limited
Depth: shallow
Stereopsis: absent (monocular)
Image: upright
View of Periphery: limited (upto equator only)
Working Distance: very close
Scleral indentation: difficult
Easier to learn

Indirect Ophthalmoscopy: smaller image but wider view
Magnification: less magnified (2 - 5X)
Field of View: wider (36 - 63 degrees)
Illumination: higher
Depth: deep
Stereopsis: present (binocular)
Image: inverted/reversed
View of Periphery: full (upto pars plana)
Working Distance: arm’s length
Scleral indentation: easy
Harder to master

19
Q

Discuss color coding in drawing retinal pathologies.

A

BLUE
- detached retina
- retinoschisis (OPL)
- lattice degeneration
- retinal veins

RED
- retinal arteries
- retinal breaks (outlined in blue)
- hemorrhages
- micro/macroaneurysms
- neovascularization

BROWN
- pigmentation
- hypopigmentation
- photocoagulation

GREEN
- vitreous opacities (hemorrhage, floaters)
- media opacities (cataract, corneal)
- periretinal fibrosis

ORANGE
- exudation
- cotton-wool spots
- chorioretinitis

YELLOW
- disc edema/pallor
- retinal edema

20
Q

What is the most common contact type lens used in slit lamp biomicroscopy?

A

Goldman 3-mirror lens
- uses three mirrors of different angulations
- for gonioscopy and laser gonioplasty
- good magnification: 0.93X
- wide field of view: 140 degrees
- disadvantage: tedious to use

Mirror 1
- circular
- not angled
- view: macula

Mirror 2
- trapezoid
- 73 degrees
- view: posterior pole to equator

Mirror 3
- rectangular
- 67 degrees
- view: equator to ora serrata

Optional: Mirror 4 (Goldman 4-mirror Lens)
- thumbnail/parabolic
- 59 degrees
- view: ora serrata to anterior chamber

21
Q

What are the other common contact type lenses used in slit lamp biomicroscopy?

A

Widest fields of view: 160 - 165 degrees, 0.50X
1. Super Quad 160
2. H-R Wide Field 160
3. Mainster PRP 165 (dynamic: upto 180 degrees)

Others:
1. Centralis Direct
- similar to Mirror 1 of Goldman 3-mirror
- Magnification: 1.0X
- View: central retina/macula

  1. Area Centralis
    - similar to Mirror 2 of Goldman 3-mirror
    - Magnification: 1.0X
    - View: posterior pole to equator (70 degrees)
    - useful for macular exam and treatment
  2. Trans Equator
    - Magnification: 0.70X
    - View: beyond the equator (110 degrees)
    - useful for panretinal photocoagulation
  3. QuadrAspheric
    - Magnification: 0.50X
    - View: upto ora serrata (120-130 degrees)
    - useful for panretinal photocoagulation
22
Q

What are common non-contact type lenses used in slit lamp biomicroscopy?

A
  1. Digital High Mag
    - M: 1.30X
    - V: 57 degrees
    - high magnification but smallest field of view
  2. 90D Classic
    - M: 0.75X
    - V: 74 degrees
    - standard diagnostic lens
  3. Super Field NC
    - M: 0.76X
    - V: 95 degrees
  4. Super Pupil XL
    - M: 0.45X
    - V: 103 degrees (upto equator); dynamic: 124 degrees
    - allows IO with a slit lamp
  5. Digital Wide Field
    - M: 0.72X (~ 90D lens)
    - V: 103 degrees (upto equator); dynamic: 124 degrees
23
Q

What are the common indications and applications for OCT?

A
  1. Anatomy and layers of the macula and fovea
  2. Macular and foveal thickness
  3. Vitreous, choroid, and vitreo-retinal interface
  4. Monitoring vitreo-retinal diseases
  5. Evaluating treatment outcomes
24
Q

What are the FOUR HYPERreflective outer retinal bands seen in the SS-OCT?

A

(DARK) Outer Nuclear Layer
(LIGHT) External Limiting Membrane : apical processes of Muller Cells
(DARK) Myoid Zone of Inner Segment
(LIGHT) Ellipsoid Zone of Inner Segment
- densely packed with mitochondria causing increased backscattering of light and high refractive index
- junction of inner segment and outer segment
(DARK) Outer Segment
(LIGHT) Interdigitation Zone: junction of outer segment and RPE
(DARK + LIGHT) RPE/Bruch’s Membrane Complex

25
Q

What layers are present in the fovea?

A

ABSENT: innermost layers: NFL, GCL, IPL, INL
PRESENT:
(LIGHT) Henle’s Layer/OPL: high conc. of CONES
(DARK) ONL
(LIGHT) ELM
(DARK) Myoid Zone: inner segment
(LIGHT) Ellipsoid Zone: junction of inner & outer segment
(DARK) Outer Segment
(LIGHT) Interdigitation Zone: junction of outer segment and RPE
(DARK + LIGHT) Retinal Pigment Epithelium

26
Q

What is Optical Coherence Tomography?

A
  • Imaging technique that uses low-coherence light to capture micron-resolution, 2D and 3D images from within an optical media
  • Multiple A scans fired in rapid succession
  • Relies on optical differences of tissues
  • Tomography: generating a 2D image of a section through a 3D object using a penetrating wave
  • Based on low-coherence interferometry using:
    1. Michelson interferometer
    2. Near-infrared light: wavelength ~ 800 nm
  • Long wavelength light: allows penetration into the scattering medium
  • Analogue: B-Mode Ultrasound
    + But uses light instead of sound
    + Light travels faster permitting > 100X greater resolution

How does it work?
1. Light source is directed towards a beam splitter which splits beam into two.
2. One beam directed into a reference mirror while the other is directed into the retina.
3. Reflections of both light waves are received by an interferometer which superimposes the two to form an interference pattern.
4. Interference pattern is analyzed by a detector and forms a 2D or 3D image of the retina.

27
Q

Compare ultrasound technology using sound waves vs OCT using light waves.

A

Ultrasound
- sound is slower
- image is blurred similar to a photo of a moving subject or with slow shutter speeds
- based on reflection of sound waves (10^7 Hz)
- (+) echo delay
- needs direct contact for propagation
- poor resolution: 150 um

OCT
- light travels faster
- faster shutter speed
- based on reflection of low-coherence light (near infrared: 800 nm ~ 10^14 Hz)
- direct contact is NOT necessary
- high resolution: < 10 um

28
Q

What is interferometry?

A
  • Technique which uses the interference of superimposed waves to extract information
  • Light from a single source is split into two beams that travel in different optical paths, which are then combined again to produce interference
  • The resulting interference fringes give information about the difference in optical path lengths

Michelson interferometer
- Usually used in OCT
- 1st interferometer arm: focused onto the tissue sample
- 2nd interferometer arm: bounced off a reference mirror
- Reflected light from the tissue sample is combined with reflected light from the reference mirror
- Low coherence light: interferometric signal observed only at a limited depth
- Records one thin optical slice of the sample at a time
- By performing multiple scans, moving the reference mirror between each scan, an entire three-dimensional image of the tissue can be reconstructed

29
Q

What are the three basic kinds of OCTs?

A
  1. Time Domain OCT: lowest resolution
  2. Spectral Domain OCT
  3. Swept Source OCT: highest resolution similar to a histologic section even at the fovea
30
Q

How does a Time Domain OCT work?

A
  • Light source: broad band of light from a super luminescent diode
  • Reference mirror: moves back and forth
  • Image: 10 um (grainy)
  • Disadvantage:
    + Mirror movement and speed determines image quality and resolution (TIME-domain)
    + Speed of movement is limited thus image is poorer in quality
31
Q

How does a Spectral Domain OCT work?

A
  • Light source: broad band of light from a super luminescent diode (similar to TD-OCT)
  • Reference mirror: FIXED
  • Utilizes the Fourier analysis: converts a signal from its original domain (often time) to a representation in the frequency domain
  • Frequency domain: analysis of signals with respect to frequency, rather than time.
  • Image: 5-7 um (better resolution than TD-OCT)
  • Added components:
    1. Diffraction Grating Detector:
  • receives interference pattern from the interferometer
  • separates the light wave into its component wavelengths
    2. Spectrophotometer or Spectrum Analyzer
  • receives light from the DGD
  • analyzes spectrum of light and forms a spectral interferogram
  • processed interferogram subjected to fast Fourier transform to form the final image
32
Q

How does a Swept Source OCT work?

A
  • Light source: Swept-Source Laser
    + Type of laser in which the output wavelength is adjustable over a wide range of wavelengths
    + Starts with a particular wavelength of light and “sweeps” across the spectrum of light with a range of wavelengths
    + Capable to producing narrow wavelength or coherent light
  • Reference mirror: FIXED (similar to SD-OCT)
  • Image: < 5 um (best resolution similar to a histologic section)
  • Deeper penetration: imaging even the choroid and sclera
  • Added components:
    1. Balanced Detector
  • receives interference pattern from the interferometer
  • diffraction grating unnecessary because wavelengths of light has already been separated at the source
    2. Spectrophotometer or Spectrum Analyzer
  • similar to a SD-OCT
  • receives light from the BD
  • analyzes spectrum of light and forms a spectral interferogram
  • processed interferogram subjected to fast Fourier transform to form the final image
33
Q

Compare properties of TD-OCT vs SD-OCT vs SS-OCT.

A

TD-OCT
Light source: 820 nm
Detector: Single
Axial resolution: 10 um
Scanning speed: 400 A-scans per second (slow)
Scanning depth: 2 mm
Retinal thickness: ILM to IS/OS (Ellipsoid Zone)
Speed of data acquisition: Slower than eye movement

SD-OCT
Light source: 840 nm (broader bandwidth)
Detector: Spectrophotometer + FFT
Axial resolution: 5-7 um
Scanning speed: 25,600 A scans per second (18,000 - 40,000)
Scanning depth: 2 mm
Retinal thickness: ILM to RPE
Speed of data acquisition: Faster than eye movement

SS-OCT
Light source: 1050 nm (range of wavelengths using a swept laser)
Detector: Spectrophotometer + FFT
Axial resolution: < 5 um (best resolution)
Scanning speed: 100,000 A scans per second
Scanning depth: 2.6 mm (deeper penetration)
Retinal thickness: ILM to choroid, sclera, LC
Speed of data acquisition: Faster than eye movement

34
Q

What is False Color Representation technology used in OCTs?

A
  • Principle of REFLECTIVITY: proportion of incident light directly backscattered by tissues

HIGH: reflects back ALL light
- Color: White, Red
- E.g.: NFL, RPE, silicone oil-retina interface, dense scar tissue

MODERATELY HIGH: reflects back MAJORITY of light
- Color: Orange, Yellow
- E.g.: Plexiform layers (IPL, OPL) and photoreceptor layer (yellow to green), scar tissue, choroidal neovascularization, hemorrhages

MODERATE: reflected and scattered light in equal proportion
- Color: Green
- E.g.: Plexiform layers (IPL, OPL) and photoreceptor layer (yellow to green), vitreous bands

MODERATELY LOW: absorbs MAJORITY of light
- Color: Blue
- E.g.: Nuclear layers (GCL, INL, ONL), choroid (green to blue), vitreous debris

LOW: absorbs or transmits ALL light
- Color: Violet, Black
- E.g.: Vitreous gel, Aqueous humor, Sclera, silicone oil, cysts

35
Q

What is OCT Angiography?

A
  • Imaging of the microvasculature of the retina and choroid
  • Uses Fourier domain (SD or SS) OCT: high imaging speed enables measurement of blood flow
  • Uses the reflective properties of the surface of moving RBCs accurately outline vessels
  • Able to distinguish slow flow in small blood vessels from biological motion in extravascular tissue
  • Examples:
    1. Split-Spectrum Amplitude Decorrelation Angiography (SSADA)
    2. Phase Variance OCT (pvOCT)
36
Q

What are the parts of an OCT report?

A
  1. Scan Information
  2. En face image: image of the surface of the fundus
  3. Topographic map with reflectivity scale: color-coded topography of the retinal surface
  4. En face with topographic overlay: combines 2 and 3
  5. Retinal thickness map
    - Normal: GREEN; within 90% of normal population (5 - 95%)
    - Thickened:
    + PINK: upper 1% (> 99%); extremely thickened
    + PALE YELLOW: upper 4% (95 - 99%); moderately thickened
    - Thinned:
    + BRIGHT YELLOW: lower 4% (1 - 5%); moderately thinned
    + RED: lower 1% (< 1%); extremely thinned
  6. B-scan Images: combination forms a 3D image
    - Horizontal
    + imaged at the 180 degree meridian
    + see NASAL and TEMPORAL retina
    - Vertical:
    + imaged at the 90 degree meridian
    + see SUPERIOR and INFERIOR retina
  7. Segmentation Layers: to assess extent or depth of lesions
37
Q

What are the steps in the basic interpretation of an OCT report?

A
  1. Assess centration of the scan on the fovea.
    - presence of foveal depression
    - absence of INNER retina layers in the fovea: NFL, GCL, IPL, INL
    - tapering of retinal layers approaching the fovea
    + appearance: hourglass or bowtie
    + NEVER lost even in pathologic conditions
  2. Assess retinal topography and thickness
  3. Evaluate integrity of layers in the B-scan image
  4. Describe macular and retinal pathologies if present
    - Parameters: location, size, depth of involvement
    -E.g. Cysts, Elevations, Depression
38
Q

OCT findings in common retinal pathologies.

A
  1. Age-Related Macular Degeneration
    - intraretinal cysts: cystic changes in the macula (hyporeflective)
    - neovascularization: thickening of choroid (hyperreflective)
    - drusen: irregularities in the RPE surface (hyperreflective)
  2. Macular Edema
    - intrarenal exudates: fluid collection in between the layers of the NSR in the macular/foveal area
    - intraretinal cysts
  3. Central Serous Retinopathy
    - fluid collection between NSR and RPE in the macular/foveal area
    - detachment of NSR from RPE
  4. Vitreo-Macular Traction
    - traction of the vitreous on the fovea causing detachment of NSR layers from the RPE
    - end-point: macular hole
  5. Macular Hole
    - full-thickness: complete absence of all layers; only RPE is present
    - lamellar/partial-thickness: partial defect with some layers present
  6. Retinal Detachment
    - detachment of NSR from RPE in the peripheral retina
    - Rhegmatogenous: due to retinal tear from traction in the vitreous base
    - Tractional: due to fibrovascular proliferation and scar formation
    - Exudative: due to fluid collection in the subretinal space, (-) tear
  7. Pigment Epithelial Detachment
    - detachment of RPE from the choroid
  8. Epiretinal Membrane
    - thick hyperreflective layer on the surface of the NSR
    - vs ILM: nondescript or a very thin hyperreflective line