Culture and Sensitivity

Question 1

What is a prokaryote?

Answer 1

Unicellular organism that lacks organelles and membrane bound structures

Question 2

What are the 4 requirements for a prokaryote to live?

Answer 2

Temperature, ph, oxygen, nutrients

Question 3

What prokaryotes need oxygen?

Answer 3

Obligate anaerobe

Question 4

What prokaryote can survive in oxygen but growth is limited?

Answer 4

Facultative anaerobes

Question 5

Which prokaryote needs oxygen, cannot ferment but will die in high amounts of oxygen?

Answer 5

Microaerophilic

Question 6

What is capnophilic?

Answer 6

Requiring high amounts of carbon dioxide.

Question 7

What is the ideal prokaryotic ph?

Answer 7

6.5-7.5

Question 8

What are 4 methods of IDing prokaryotes?

Answer 8

Size, shape (coccus, bacillus, spirilli, pleomorphic), arrangement (single, pairs, chains, tetrads, clusters, palisades), chemical reactivity

Question 9

What are the 4 stages of bacterial growth?

Answer 9

Initial, exponential, stationary, final

Question 10

What are fungi?

Answer 10

Filamentous strands of cells that secrete enzymes and feed on the organic material they grow on.

Question 11

Fungi are either:

Answer 11

parasitic or saprophytic (uses decaying material as energy)

Question 12

How does yeast reproduce?

Answer 12

Asexual budding

Question 13

Which need living cells to survive, virus or bacteria?

Answer 13

Virus

Question 14

What is selective grow media and an example?

Answer 14

Allows one specific bacteria to grow. MacConkey agar

Question 15

What is enrichment grow media?

Answer 15

Inhibits growth of some organisms while promoting likely pathogens

Question 16

What is nutrient rich grow media?

Answer 16

Allows most bacteria to grow, blood agar

Question 17

What is differential grow media?

Answer 17

Contains indicator that allows user to ID and differentiate organisms

Question 18

Which agar contains both differential and enrichment media?

Answer 18

Blood agar

Question 19

What is the most common agar?

Answer 19

Trypticase soy agar with sheep blood

Question 20

What are the 4 types of hemolysis? Describe each.

Answer 20

Alpha, beta, gamma, delta. Alpha is partial hemolysis with green/slimy discoloration. Beta is complete hemolysis with a clear zone around the colony. Gamma is hemolysis that produces no change in medium. Delta has double zone hemolysis, both alpha and beta

Question 21

MacConkey medium grows what?

Answer 21

Gram negative bacteria. Lactose producing bacteria turn pink.

Question 22

If there is growth on MacConkey but growth on Blood agar, what bacteria is growing?

Answer 22

Gram positive

Question 23

THIOGLYCOLLATE BROTH is a common liquid medium used for which bacteria?

Answer 23

Anaerobic

Question 24

Triple sugar iron is used to grow which bacteria?

Answer 24

Gram negative enterobacteria like salmonella, e.coli, which ferment glucose and become acidic

Question 25

What does SLANT and BUTT detect?

Answer 25

SLANT detects utilization of lactose and sucrose, BUTT detects glucose fermentation

Question 26

In the triple sugar iron test, acidic and alkaline changes are what color?

Answer 26

Acidic are yellow, alkaline are red

Question 27

How are agar plates/jars disposed of?

Answer 27

Add dilute bleach or chlorhex, seal container and let sit 4-5 minutes, then trash.

Question 28

What things are the most commonly cultured?

Answer 28

Blood, urine, spinal fluid, joint fluid, milk, solid organs, respiratory tract