Cultivation of Viruses Flashcards
T/F: Outside the host, viruses are inert/dormant
True
Do viruses have the genetic capability to multiply by division?
No
What do viruses need to replicate and generate the next progeny of viruses?
Living host cell
What are the 3 possible ways to grow/culture viruses?
- Cell/tissue culture
- Inoculation in embryonated egg
- Laboratory animals
What is the culture that has cells attached to the plate surface?
Monolayer cultures
What is a primary cell culture?
Maintenance of growth of cells dissociated directly from parental tissue of human or animal origin
What are two advantages of primary cell culture?
- Best culture system for isolation and propagation of viruses
- Used to produce viral vaccines
What is a subculture?
Transfer of cells from one culture vessel to another culture vessel
Why would a subculture be needed?
To provide fresh nutrients and growing space for continuously growing cell lines
What two categories are cell lines placed into?
- Finite/diploid cell lines
2. Continuous cell lines
Finite/diploid cell lines have a _______ life span
Limited
Where are finite/diploid cell lines derived from?
Mainly from embryos; or from secondary cell cultures
T/F: Finite/diploid cell lines can be used for vaccines
True
Where are continuous cell lines derived from?
Directly from cancer cells
T/F: Continuous cell lines are used in vaccine production
False; FDA prohibits their use in vaccine production
