Core Practicles Flashcards
How to view a specimen using a light microscope?
Step 1
Your specimen needs to let light through it so it needs to be a thin slice
How to view a specimen using a light microscope?
Step 2
Take a clean slide
Use tweezers to place your specimen on the slide
How to view a specimen using a light microscope?
Step 3
Add a drop of stain if your specimen is completely transparent or colourless
To make the specimen easier to see
How to view a specimen using a light microscope?
Step 4
Place a cover slip onto the specimen with no air bubbles
Place it onto stage
How to view a specimen using a light microscope?
Step 5
Select the lowest powered objective lens
How to view a specimen using a light microscope?
Step 6
Adjust the focus until you get a clear image
Total magnification formula
Total magnification
=eyepiece lens magnification x objective lens magnification
Formula for magnification
Magnification = image size
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Real size
Experiment:the of pH on enzyme activity
Step 1
Put a drop of iodine into every well of a spotting tray
Experiment:the of pH on enzyme activity
Step 2
Set up a Bunsen burner
Put a beaker if water and heat till 35°C
Experiment:the of pH on enzyme activity
Step 3
Add 3cm3 of amylase solution and 1cm3 of buffer solution with a pH of 5 to a boiling tube
Place it int he water and wait 5 mins
Experiment:the of pH on enzyme activity
Step 4
Add 3cm3 of starch solution to the boiling tube
Experiment:the of pH on enzyme activity
Step 5
Immediately mix the contents of the boiling tube and start a clock
Experiment:the of pH on enzyme activity
Step 6
Use continuous sampling to record how long it takes for the amylase to break down all of the starch
To do this: dropping pipette to get a fresh sample from the boiling tube every 10 seconds
When the iodine solution remains brown/orange the starch isn’t present
Experiment:the of pH on enzyme activity
Step 7
Repeat the experiment but with a buffer solution with different pH values to see the effect of pH
