CORE PRACTICAL 6

Question 1

What is core practical 6? (1)

Answer 1

determining the water potential of a plant tissue.

Question 2

what is the first step of this practical? (2)

Answer 2

take a potato and cut 6 cylinders, 4cm long using a cork borer. make sure none of the cylinders have peel attached. blot each cylinder carefully to remove excess liquid and weight it.

Question 3

what is the second step of this practical? (1)

Answer 3

label 5 large boiling tubes one with distilled water, 0.25, 0.5, 0.75 and 1.0.

Question 4

what is the third step of this practical? (2)

Answer 4

make up 30cm3 of these concentrations of sucrose solution. place one cylinder in each boiling tube and add 30 cm3 of the appropriate solution to each tube.

Question 5

what is the fourth step of this practical? (3)

Answer 5

leave all of the boiling tubes and their contents in a cool place for 2 hours. after this time carefully pour out the liquid and blot the cylinders. reweigh the dried cylinders and record the new mass taking away from the original mass.

Question 6

what is the fifth step of this practical? (2)

Answer 6

calculate the percentage change in mass for each cylinder and plot a graph of percentage change on the vertical side and osmotic potential on the horizontal.