Conversion of Muscle to Meat (Exam 4) Flashcards
What is homeostasis?
Maintenance of the physiological state
What lies under the narrow range of physiological conditions?
pH
Temperature
Oxygen concentration
Energy supply
What makes the beginning of postmortem changes?
Drop in blood pressure
Homeostatic control
What are some homeostatic control mechanisms? (4)
Protective/survival mechanism
Increase in heart rate
Peripheral vessels constrict
50% blood remaining in vital organs
When is muscle converted to meat?
When metabolism stops
What happens when the oxygen supply is depleted?
TCA cycle and electron transport start failing
What does homeostatic maintenance do when oxygen is depleted?
Shifts to anaerobic metabolism
What accumulates when there is a decline of muscle pH?
Lactic acid
What is the most significant postmortem change?
Accumulation of lactic acid
What is the normal decline pattern of postmortem pH?
Gradually from 7.0 to 5.7 within 6 to 8 hours postmortem
What is the ultimate pH postmortem?
5.5 within 24 hours
What are some factors to postmortem temperature change?
Rate of metabolism (heat production) Size and location of muscles Predominate muscle fiber type Duration of metabolism Fat insulation Slaughter: handling, stress, stun/stick
What are 2 sources of ATP postmortem?
Glycolytic/anaerobic metabolism
Creatine phosphate
What sets in when ATP depletes?
Rigor
What is rigor mortis?
Stiffness of death
What are the phases of rigor mortis?
Delay phase
Onset phase
Completion phase
When does the delay phase occur?
Immediately after exsanguination
How many actomyosin cross bridges are there during delay phase?
Few
When does the onset phase occur?
Within hours, species-dependent
What happens during onset phase? (4)
Less and less phosphorylation of ADP to ATP
Sources of ATP decreased
More actomyosin cross-bridges
Gradually less extensible
What is the ATP supply like in the completion phase?
Exhausted because glycolysis stops and creatine phosphate depletes
Describe the completion phase.
Maximum actomyosin cross-bridges All binding sites used Sarcomeres shortened Muscle tension to maximum Actomyosin bonds cannot be broken
Look at postmortem graph
Look at postmortem graph
How many binding sites are used in rigor?
100%
How many ending sites are used in normal contraction?
20%
What is the resolution of rigor mortis?
Decrease in tension with time postmortem
What happens in resolution of rigor? (4)
Proteolytic degradation
Z disk dissolution
Loss of ultrastructural integrity
Actomyosin bonds still not broken
When does the most notable change occur?
48-72 hours postmortem
What does the most notable change cause?
Increased tenderness and aging effects
What is involved in the degradation of z disks?
Desmin
What is involved in the decrease in passive elasticity of muscle?
Titin
What is rigor tension?
Rupturing Z line/ I band junction
What happens during Z disk degradation?
Rigor tension
Decreases elasticity of myofibrils
What enzymatic activities cause resolution of rigor?
Postmortem proteolytic degradation of myofibril proteins
Calpain system