Controlling Microbial ( micro para Flashcards

1
Q

FACTORS THAT AFFECT
MICROBIAL GROWTH

A

Availability of Nutrients
Moisture
Temperature

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2
Q

Microorganisms that grow best at high temperatures are
called

A

thermophiles

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3
Q

Organisms that favor
temperatures above 100°C are referred to as

A

hyperthermophiles

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4
Q

Most microorganisms prefer a what ph

A

neutral or slightly
alkaline growth medium (pH 7.0–7.4)

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4
Q

The highest
temperature at which a bacterium has been found living is
around 113°C; it was an archaeon named

A

Pyrolobus
fumarii.

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5
Q

Microbes that grow best at moderate temperatures are
called

A

mesophiles

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6
Q

prefer cold temperatures.

A

Psychrophiles

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7
Q

prefer warm temperatures
but can endure very cold or even freezing
temperatures.

A

Psychroduric organisms

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8
Q

prefer a pH of >8.5

A

– Alkaliphiles

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8
Q

prefer a pH of 2 to 5

A

– Acidophiles

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9
Q

is the movement of a solvent, through a
permeable membrane, from a lower concentration of
solutes (dissolved substances) to a higher
concentration of solutes.

A

Osmosis

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9
Q

is the pressure that is exerted on a
cell membrane by solutions both inside and outside
the cell

A

Osmotic pressure

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10
Q

When the concentration of solutes in the external
environment of a cell is greater than that of solutes
inside the cell, the solution in which the cell is
suspended is said to be

A

hypertonic.

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10
Q

is a condition in which the cell membrane
and cytoplasm of a cell shrink away from the cell
wall; it occurs when bacteria with rigid cell walls are
placed in a hypertonic solution.

A

Plasmolysis

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10
Q

When the concentration of solutes outside a cell is
less than that of solutes inside a cell, the solution in
which the cell is suspended is said to be

A

hypotonic.

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11
Q

If a bacterial cell is placed into a hypotonic solution,
it may not burst (because of the rigid cell wall). If it
does burst, the cytoplasm escapes; this process is
known as

A

plasmoptysis.

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11
Q

A solution is said to be ___ when the
concentration of solutes outside a cell equals the
concentration of solutes inside the cell.

A

isotonic

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12
Q

Organisms that prefer to live in salty environments
are called

A

halophilic organisms.

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13
Q

Those that do not
prefer to live in salty environments but are capable
of surviving there (e.g., Staphylococcus aureus) are
called .

A

haloduric organisms

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14
Q

Microbes that can survive in high atmospheric
pressure (>14.7 psi) are known as

A

piezophiles.

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15
Q

prefer the same atmosphere that
humans do (~20%–21% O2 and 78%–79% N2, and
<1% other gases)

A

Obligate aerobes

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16
Q

require reduced concentrations of
oxygen (~5% O2).

A

Microaerophiles

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17
Q

require increased concentrations of CO2
(5%–10% CO2).

A

Capnophiles

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17
Q

are killed by the presence of
oxygen

A

Obligate anaerobes

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18
Q

The time it takes for one cell to become two cells is
called the

A

generation time

19
Q

are used in microbiology
laboratories to culture (i.e., grow) bacteria

A

media (sing., medium)

20
Q

A ___ defined medium is one in which all
ingredients are known.

A

chemically

21
Q

an ___ is a broth or solid containing a rich
supply of special nutrients that promote the growth of
fastidious organisms, for example, chocolate agar

A

enriched medium

22
Q

A ___ has added inhibitors that discourage
growth of certain organisms while allowing the growth of
a desired organism, for example, PEA agar.

A

selective medium

23
Q

A ___ permits the differentiation of
organisms that grow on the medium, for example,
MacConkey agar

A

differential medium

24
Q

is a popular liquid medium in
bacteriology laboratories; it supports the growth of all
categories of bacteria from obligate aerobes to obligate
anaerobes.

A

Thioglycollate (THIO) broth

25
Q

Unwanted organisms are referred to as ____;
the growth medium or plate is said to be contaminated

A

contaminants

26
Q

After media are inoculated, they must be placed into an
incubator which will maintain the appropriate
atmosphere, temperature, and moisture level; the
process is known as

A

incubation

27
Q

Three types of incubators are used in clinical
microbiology laboratories

A

– A CO2 incubator (contains 5%–10% CO2)
– A non-CO2 incubator (contains room air)
– An anaerobic incubator (the atmosphere is devoid of
oxygen)

28
Q

A ___ can be used to determine growth
by measuring the turbidity of the medium.

A

spectrophotometer

29
Q

are microbes that can
survive and multiply only within living cells (called host
cells).

A

Obligate intracellular pathogens

29
Q

A _____ is used to determine the number of
viable bacteria in a liquid sample by making serial
dilutions of the liquid and inoculating onto nutrient agar;
after overnight incubation, the number of colonies is
counted.

A

viable plate count

30
Q

are solutions used to disinfect skin and
other living tissues.

A

Antiseptics

31
Q

Examples of protozoa that can be cultured in vitro are

A

amebae, Giardia lamblia, Leishmania spp., Toxoplasma
gondii, Trichomonas vaginalis, and Trypanosoma cruzi

32
Q

is the destruction or removal of pathogens
from nonliving objects by physical or chemical methods;
pasteurization is an example of a disinfection technique.

A

Disinfection

32
Q

is the complete destruction of all microbes,
including cells, spores, and viruses

A

Sterilization

32
Q

are chemical substances that eliminate
pathogens on inanimate objects

A

Disinfectants

33
Q

The suffix -cide or -cidal refers to

A

“killing.”

33
Q

agents kill bacterial endospores

A

Sporicidal

34
Q

agents kill fungi, including fungal spores.

A

Fungicidal

35
Q

agents kill algae.

A

Algicidal

36
Q

agents destroy viruses.

A

Viricidal

37
Q

A ___ is a drug or chemical that inhibits
growth and reproduction of microbes

A

microbistatic agent

38
Q

A ____ is one that specifically inhibits the
metabolism and reproduction of bacteria.

A

bacteriostatic agent

39
Q

is a process that combines dehydration
(drying) and freezing. this process is widely used in
industry to preserve foods, antibiotics, microorganisms,
and other biologic materials.

A

Lyophilization

40
Q

refers to the presence of pathogens in blood or
tissues

A

Sepsis

40
Q

whereas ___ means the absence of
pathogens.

A

asepsis

41
Q

is the prevention of infection.

A

Antisepsis

42
Q

The _____ of any species is the
lowest temperature that will kill all of the organisms
in a standardized pure culture within a specified
time

A

thermal death point (TDP)

43
Q

A large metal pressure cooker that uses steam under
pressure to completely destroy all microbial life

A

autoclave

44
Q

—most microorganisms are not killed, but their
metabolic activities are slowed

A

Cold

45
Q

—many dried microorganisms remain viable,
but they cannot reproduce.

A

Desiccation

46
Q

used in hospitals and medical and
dental clinics to clean equipment.

A

Ultrasonic waves

46
Q

an ultra-violet (UV) lamp is useful for
reducing the number of microbes in the air.

A

Radiation

47
Q

used to separate cells/microbes from liquids or
gases.

A

Filters

48
Q

—can be altered to inhibit growth.

A

Gaseous atmosphere