Connective Tissue Flashcards

1
Q

Where is the origin of connective tissue cells

A

Mainly from undifferentiated mesenchymal cells

Haematopoietic stem cell line

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2
Q

What are the constituents of connective tissue

A

Cellular component
- fibroblasts/cytes
- adipose tissue

Extracellular components
- visible fibres
- ground substance (hydrophilic jelly) - doesn’t stain with H&E appears white spaces

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3
Q

What are the types of connective tissues

A
  1. Fibrous (depending on collagen fibres within them)
    - Loose
    - Dense
  2. Hard
    - Cartilage
    - Bone
  3. Fatty
    - White (most prevalent of the fats)
    - Brown (role in thermogenesis)
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4
Q

What is collagen

A

Secreted by fibroblasts as tropocollagen - triple helix of peptides

Fibres assembled extra-cellularly

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5
Q

How many types of collagen are there

A

More than 20

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6
Q

What is type 1 collagen prevalent in

A

Skin and bone

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7
Q

What is type 2 collagen prevalent in

A

Cartilage

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8
Q

What is type 3 (reticulin) collagen prevalent in

A

Liver, bone marrow, spleen

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9
Q

What is type 4 collagen prevalent in

A

Basement membrane

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10
Q

What is type 5 collagen prevalent in

A

Placenta

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11
Q

What colour do collagen fibres stain with H&E

A

Extracellular - pink

Variable thickness and length - often run in bundles

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12
Q

What is the significant difference between collagen fibres and muscle fibres

A

Muscle fibres are intracellular (nuclear lies within them)

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13
Q

What does loose connective tissue comprise of

A

Widely spaced thin collagen fibres

Fibroblasts/fibrocytes

Unstained ground substances

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14
Q

What does dense connective tissue comprise of

A

Closely spaced thick collagen fibres

Fibroblasts/fibrocytes

Unstained ground substance (less abundant than in loose connective tissue)

May be irregular or regular (running parallel to each other)

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15
Q

What is the role of type 3 collagen - reticulin

A

Fibrillar collagen

Forms a supporting scaffold in many organs
- Bone marrow
- Liver/kidney
- Lymph node
- Spleen

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16
Q

What stain must be used to see reticulin

A

Silver stain

Not visible on H&E

17
Q

What is elastin (elastic tissue)

A

Contains fine fibres and sheets of elastin

Elastin fibres may be branched

Produced by fibroblasts

18
Q

What colour is elastin stained on H&E

A

Pink

19
Q

What is white adipose (fatty) tissue

A

Large cells with single fat globule in each cell

Usually appear empty (white) in conventional slides as fat is extracted during process

20
Q

What is brown adipose (fatty) tissue

A

Cells with many globules of fat

Found across shoulders and down back of newborn

Important in neonatal thermo-regulation (generate heat to breakdown)

21
Q

What are the constituents of cartilage

A

Hard connective tissue

2 cell types
- chondroblasts
- chondrocytes

Set in abundant extracellular matrix

Extracellular fibres

Avascular - gets blood from blood vessels either side of cartilage

22
Q

What are the three types of cartilage

A

Hyaline cartilage

23
Q

What is hyaline cartilage

A

Found in synovial joints

Chondrocytes in lacunae surrounded by a glassy amorphous matrix

No visible fibres

Perichondrium - fibroblasts and collagen

24
Q

What is elastic cartilage

A

Visible elastic fibres

Perichondrium - fibroblasts and collagen

Chondrocytes trapped within matrix

Elastic fibres easily visible on H&E but clear on silver stain

25
Q

Where is elastic cartilage found

A

Pinna
Epiglottis

26
Q

What is fibrocartilage

A

Visible collagen fibres in matrix

Perichondrium - fibroblasts and collagen

Trapped chondrocytes

27
Q

Where is fibrocartilage found

A

Annulus fibrosus

Pubic symphysis

28
Q

What is synovium

A

Lines the inside of the joint capsule

1-4 layers of synovial fluid

Two types of cells -
Type A - phagocytes
Type B - rich in rER - secrete synovial fluid

Variable shapes - squamous to cuboidal

Richly vascular, highly innervated

29
Q

What are the three categories of muscle

A

Visceral (smooth) muscle

Voluntary (skeletal) muscle

Cardiac muscle

(other)

30
Q

What are visceral (smooth) muscle

A

Arterial wall
Wall of intestine
Airways of lungs

31
Q

What are voluntary (skeletal) muscle

A

Skeletal muscle
Larynx
Diaphragm

32
Q

What are cardiac muscle

A

Heart
Base of great vessels

33
Q

What fall under other muscle types

A

Pericytes
Myo-fibroblasts
Myo-epithelial cells

34
Q

What is smooth muscle

A

Involuntary muscle

Fusiform cells

Central nucleus

Non striations

Non-branching

35
Q

Describe the features of the dermis underneath the epidermis

A

Thick layer of dense irregular connective tissue

Consists of long fibres of collagen that run in many directions to form a mat-like fibrous scalp

Fibres stain dark pink

Have fibroblasts with dark blue nuclei lying alongside them

36
Q

What are tendons composed of

A

Thick ribbons of parallel collagen fibres

Between lie the fibroblasts which synthesis them

37
Q

Describe the composition of the penis

A

Contains mixture of loose and dense irregular fibrous connective tissue

38
Q

Describe the wall of arteries

A

Most contain a large amount of smooth muscle

Those close to the heart the muscle replaced by concentric sheets of elastic tissue

Sheets stain pink with eosin