Compatibility Testing

Question 1

Consist of mixing donors serum to patients red cell

Answer 1

Minor cross matching

Question 2

True or false it is necessary to perform a minor cross match because donor units are screened for unexpected antibodies

Answer 2

False it is no longer perform

Question 3

Give the 6 causes of positive results in the major crossmatching

Answer 3

1. Incorrect ABO grouping of the patient or donor
2. An alloantibody in patients serum reacting with the corresponding antigen on donor cell
3. An autoantibodies in patients serum reacting with the corresponding antigen on donor cell
4. Prior coating of the donor cell with proteins , resulting in positive AGT
5. Abnormalities in patients serum
6. Contaminants in the test system

Question 4

A method for major crossmatch test type and screen coupled with and immediate crossmatch

Answer 4

Abbreviated crossmatch

Question 5

Name the 4 methods in major crossmatching

Answer 5

Immediate spin crossmatch
Abbreviated crossmatch
Electronic/computer crossmatch
Antiglobulin crossmatch

Question 6

A method for major crossmatch test that is accomplished by simply mixing a patient serum with donor cells and centrifuging immediately.

Answer 6

Immediate spin crossmatch

Question 7

A method for major crossmatch test that compares recent ABO serologic results and interpretation on file for both the donor and the patient being matched and determine compatibility based on this comparison.

Answer 7

Electronic crossmatch

Question 8

This procedure begins at the same manner as the immediate spin crossmatch continues at 37*C incubation and finishes with AGT

Answer 8

Antihuman crossmatch

Question 9

In immediate spin crossmatch absence of hemolysis agglutination indicates?

Answer 9

Compatibility

Question 10

Compatibility testing protocol

Answer 10

Testing of donor sample

Testing of patient sample

Question 11

Compatibility testing consist of the ff.

Answer 11

Review of patients past blood bank history and records.
ABO and RH grouping of the recipient and donor.
Antibody screening of the recipient and donor’s serum.

Question 12

Used to detect antibodies bound to erythrocyte in vivo.

Answer 12

Direct globulin test

Question 13

Used to detect the reaction of the antibodies and erythrocyte in vitro after an appropriate incubation phase

Answer 13

Indirect globulin test

Question 14

What are the application of direct anti globulin test?

Answer 14

HDN
HTR
Autoimmune and drug induced hemolytic anemia

Question 15

AGT is commonly called?

Answer 15

Coomb’s test

Question 16

What are the factors affecting anti globulin test?

Answer 16

Ratio of serum to cell
Reaction medium
Temperature
Incubation time
Washing of cells
Saline for washing
Addition of anti human globulin 
Centrifugation for reading

Question 17

Consist of mixing patient serum to donor cell

Answer 17

Major cross match