CIS PHARM

Question 1

Agents that affect the nerve action potential?

Answer 1

• Tetrodotoxin
• Local anesthetics

Both block voltage-gated Na channels

Question 2

Agents that affect vesicular acetylcholine release?

Answer 2

• Botulinum toxin
  
  • cleaves components SNARE no release ACh
• Tetanus Toxin
  
  • no fusion by target synaptobrevin
  • will block release inhib neurotransmitters

Question 3

Agents that affect depolarization?

Answer 3

Neuromuscular blocking drugs

Agonists or antagonists

• Carare alkaloids (d-tubocurarine)
• Succinylcholine

Question 4

agents that affect hydrolysis of ACh by AChE

Answer 4

Acetylcholinesterase inhibitors

Question 5

agents that affect muscle contraction

Answer 5

• Dantrolene

Question 6

What is the rate limiting step in ACh biosynthesis?

Answer 6

Uptake of choline by choline transporter

Question 7

Process of vesicle-membrane fusion?

Answer 7

SNARE proteins mediate vesicle fusion

• v-SNARE= Synaptobrevin= VAMP
• t-SNARE=SNAP-25
• These two come together to form core SNARE complex
  
  • bring vesicle and presynaptic membranes into close contact
• Synaptotagmin- Ca sensor triggers fusion