Chromatography RP Flashcards
Paper chromatography
Stationary phase is paper, mobile is solvent
Amount of time in each phase affected by: how soluble in solvent, how attracted to the paper
Higher solubility, less attracted, higher up paper
Chromatography results
Called a chromatogram
The r1 value allows you to see what is dissolved in it based on the r1 value of pure substances to compare
R1 value is dependent on the solvent
If they only have the same r1 in some solvents, it isn’t present
Method
Draw a line in pencil at the bottom of a sheet of filter paper
Add a spot of ink and put the paper in a beaker of solvent
Make sure the ink isn’t touching the solvent - only some dissolve in water
Place a lid on
Each dye separates as it seeps up the paper, insoluble don’t move
Take the paper out and dry it when it’s nearly at the top
Rf value
Distance travelled by substance/ distance travelled by solvent