chromatography Flashcards
what can be said about Rf if the sample does not dissolve
it is 0
how is Rf used
by comparing the experimental results to known values
what can Rf be affected by
temp of solvent
- the type of solvent used
- medium of experiment
what is TLC
thin layer chromatography
what is the most desired result in chromatography
max separation
what do polar molecules dissolve in
polar solvents
what type of bonds does ethyl ethanoate have
permanent dipole
does water or ethanol have stronger hydrogen bonds
water
the weaker the intermolecular forces….
the less polar it is
what are some examples of non-polar solvents
heptane
octane
petrol
what do non-polar molecules dissolve in
non polar solvents
what is the purpose of the lid in TLC
to contain any evaporated solvent
what is the purpose of the solvent soaked filter paper in TLC
- placed on the inside of the beaker
ensures the solvent doesn’t evaporate easily
what are the methods of identifying dots
- UV
-Ninhydrin to produce purple/pink dots - iodine crystals ( let it evaporate and a series of brown dots will appear- must wear gloves so not to contaminate the sample