Chapter 6: Inhibition Flashcards by Molly Gallagher | Brainscape

Q

suicide inhibitor

A

forms covalent bond w/ enzyme

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Q

competitive inhibitor

A

TS analog (resembles substrate)

binds enzyme active site reversibly (noncovalently)

outcompeted by adding a ton of substrate

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Q

uncompetitive inhibitor

A

binds ES complex at site other than active site

cannot be outcompeted by adding more substrate

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Q

mixed inhibitor

A

can bind enzyme active site and ES complex

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Q

competitive rate reaction & vmax/km

A

Vo = (Vmax[s]) / (alphaKm + [s])

vmax of enzyme unchanged

Km increased (more s needed to reach same Vo)

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Q

uncompetitive rate reaction & vmax/km

A

Vo = (Vmax[s]) / (Km + alpha[s])

vmax of enzyme decreased

Km decreased

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Q

mixed km

A

km either increases or decreases

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Q

what is alphaKm

A

apparent Km

1 + [I]/Ki

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Q

for uncompetitive at high [s]

A

if [s]&raquo_space; Km then Vo = Vmax / alpha’

inhibition cannot be overcome by high [s]

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Q

for uncompetitive Km to equal [s] at 1/2 Vmax

A

[s] = alpha’[s] at Km

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