Chapter 6: Enzymes Flashcards
1
Q
enzymes catalyze rxns in an ____
A
active site
2
Q
info about active sites
A
3D cleft formed by multiple parts of aa sequence
H2O usually excluded unless a reactant
determines substrate specificity
3
Q
binding interactions between the active site and enzyme are maximized in the ____
A
transition state
4
Q
types of catalysis
A
acid/base
covalent
metal ion/cofactor
5
Q
acid/base catalysis aas
A
K, D, E, H, R, S, T, C
6
Q
covalent catalysis
A
covalent enzyme substrate intermediate
7
Q
metal ion/cofactor catalysis
A
active site binds cofactor that catalyzes rxn
8
Q
4 things needed for catalysis
A
nucleophile
t.s. stabilizer
orientation clamp
specificity
9
Q
what does a protease do
A
catalyze hydrolysis of peptide bonds
works at pH 7, 1/2 life is 1000 yrs
