Chapter 44-Mycology

Question 2

Fungal agents of importance

Answer 2

Cutaneous mycotic organisms- dermatophytes

Question 3

Most commonly seen dermatophytes

Answer 3

M. canis, M. gypseum, and T. mentagrophytes

Question 4

Anthropophilic

Answer 4

confined to humans

Question 5

Zoophilic

Answer 5

parasites of animals

Question 6

Geophilic

Answer 6

free-living saprophytes in soil

Question 7

Most dermatophytes will grow on

Answer 7

the hair shaft and some can be visualized microscopically

Question 8

Dermatophyte testing

Answer 8

Pluck hairs-periphery lesion, place on slide w/ 1-2 drops of 10% KOH, after 2-10 minutes small globular arthrospores attached to the shaft are visible

Question 9

Contraindication with wood lamps

Answer 9

only 50% of cases show bc they must be at the right life stage

Question 10

Dermatophyte test medium (DTM) turns _ in the presence of dermatophytes

Answer 10

red

Question 11

Can be used in conjunction w/ DTM to accelerate formation of macroconidia used for ID and confirmation

Answer 11

Rapid sporulation medium (RSM) and Enhanced sporulation medium (ESM)

Question 12

Sabouraud dextrose agar also promotes

Answer 12

early formation of macroconidia but no color indicator

Question 13

Sample collection

Answer 13

clean lesion, collect samples from lesion periphery, incubate at room temp w/ loose cap

Question 14

Test results

Answer 14

Red color in DTM,Teal in ESM or other media, must be supported by microscopic exam

Question 15

Cultures of nondermatophytes are plated on

Answer 15

blood agar or Sabouraud dextrose agar

Question 16

Expect _ and _ in fungal cultures

Answer 16

Blastomyces and Histoplasma spp.

Question 17

Follow _ for complete ID of fungal cultures

Answer 17

flowcharts

Question 18

With fungal cultures, after incubation check for

Answer 18

spores