Chapter 43- Additional Testing

Question 1

Common methods for testing for motility

Answer 1

Hanging drop prep, wet prep, and motility media

Question 2

Wet prep is examined with a __ objective and look for

Answer 2

high dry; cells moving back and forth

Question 3

Brownian movement

Answer 3

not true motility- shifting of cells w/ little movement related to other cells

Question 4

Motility media is used if

Answer 4

organism are nonmotile on microscopic exam

Question 5

In motility media, if growth is limited to stab line,

Answer 5

nonmotile

Question 6

Diffuse growth throughout motility media means

Answer 6

motile

Question 7

Motility media uses two tubes of

Answer 7

sulfide indole motility (SIM)

Question 8

SIM is not recommended for organisms that produce

Answer 8

hydrogen sulfide bc it blackens

Question 9

Catalase test is performed on

Answer 9

gram-positive cocci and small gram-positive bacilli

Question 10

How to determine catalase positive on catalase test

Answer 10

there will be bubbles, no bubbles=negative

Question 11

Coagulase

Answer 11

an enzyme that coagulates plasma; “clumping factor”

Question 12

Coagulase test performed on

Answer 12

catalase- positive, gram positive cocci

Question 13

Two versions of coagulase test

Answer 13

Slide coagulase (rapid screening) and Tube coagulase

Question 14

Positive result on coagulase test looks like

Answer 14

clot formation

Question 15

Oxidase activity depends on the presence of

Answer 15

cytochrome C oxidase in bacteria

Question 16

Positive oxidase activity test produces

Answer 16

purple within 60 seconds

Question 17

Mastitis may be caused by

Answer 17

bacterial or mycotic organisms

Question 18

California Mastitis test is based on

Answer 18

gel formation when test reagent reacts with DNA in somatic cells

Question 19

_mL of milk placed in each cup and _ amount of reagent is added and swirled for 10 seconds

Answer 19

2; equal

Question 20

Grade results of Mastitis test

Answer 20

negative, trace, 1, 2, 3