Chapter 40-Staining specimens

Question 1

Most common stains

Answer 1

Gram stain, Ziehl-Neelsen (acid-fast) stain

Question 2

Gram staining determines

Answer 2

appropriate medium and antibacterials

Question 3

Typical stain used for yeasts

Answer 3

crystal violet and methylene blue

Question 4

Lactophenol cotton blue confirms ID of

Answer 4

fungal organisms

Question 5

Standard hematology stains shouldn’t be used for evaluating

Answer 5

bacteria

Question 6

Gram positive and negative is based on

Answer 6

cell wall structure

Question 7

Gram-positive color

Answer 7

purple

Question 8

Gram-negative color

Answer 8

red/pink

Question 9

Process of gram staining

Answer 9

Heat fix
Primary stain (crystal violet)
Mordant (iodine)
Decolorizer (ethanol)
Counterstain (safranin)

Question 10

Gram-variable

Answer 10

when an organism stains both gram positive and negative

Question 11

Potassium hydroxide (KOH) test determines

Answer 11

the true gram status

Question 12

with KOH test, gram negative develops a

Answer 12

mucoid appearance and produce a sticky strand

Question 13

with KOH test, gram positive mixture is

Answer 13

Homogeneous

Question 14

Ziehl-Neelsen stain primarily used to detect

Answer 14

acid-fast organism of Mycobacterium and Nocardia species

Question 15

Ziehl Nelson stain steps

Answer 15

Primary stain, Decolorizer, Counterstain

Question 16

with Ziehl-Neelsen stain, if stain isn’t removed by acid alcohol then the organism is

Answer 16

acid fast and appears red

Question 17

Giemsa stain is used to detect

Answer 17

spirochetes and rickettsiae