Chapter 38-Equipment & Supplies

Question 1

All specimens should be treated as potentially

Answer 1

zoonotic

Question 2

Culturette consists of

Answer 2

a rayon swab in transport media

Question 3

Culture media

Answer 3

any material solid or liquid that can support the growth of microorganisms

Question 4

Agar is

Answer 4

dried extract of sea algae

Question 5

Gelatin is

Answer 5

a protein obtained from animal tissues

Question 6

Transport media

Answer 6

keep microbes alive but do not encourage growth

Question 7

Enriched media

Answer 7

meet requirement of fastidious pathogens

Question 8

Selective media

Answer 8

contain antibacterial substances

Question 9

Differential media

Answer 9

allow bacteria to differentiate into groups by biochemical reactions

Question 10

Enrichment media

Answer 10

favor growth of a particular group of organism

Question 11

What type of media is blood agar

Answer 11

enrichment and differential

Question 12

Alpha hemolysis

Answer 12

partial hemolysis creates a band of greenish or slimy discoloration around the colony

Question 13

Beta hemolysis

Answer 13

complete hemolysis creates a clear zone around the colony

Question 14

Gamma hemolysis

Answer 14

hemolysis that produces no change in the appearance of the medium

Question 15

Delta hemolysis

Answer 15

double zone hemolysis

Question 16

What type of media are MacConkey Agar and Eosin-Methylene Blue Agar

Answer 16

selective and differential

Question 17

Lactose producing bacteria grow as

Answer 17

pinkish-red colonies

Question 18

Nonlactose fermenting bacteria produce

Answer 18

colorless colonies

Question 19

Inoculation of both blood and MacConkey agar can yield

Answer 19

information

Question 20

No growth on MacConkey but good growth on blood agar suggests

Answer 20

gram-postive bacteria

Question 21

Chromogenic agar provide sufficient information to definitively identify

Answer 21

some bacterial species

Question 22

Thioglycollate broth is a liquid medium used to culture

Answer 22

anaerobic bacteria to determine oxygen tolerance of microbes

Question 23

In thioglycollate broth, obligate aerobes will only grow in the _ layer and obligate anaerobes grow in the _ portion

Answer 23

top; lower

Question 24

If bacteria hydrolyze, the urea in the medium, ammonia production turns the medium

Answer 24

pink

Question 25

How to inoculate Sulfide-Indole Motility Tubes

Answer 25

a straight stab to approximately 1in deep, draw wire out same line as entry

Question 26

in Sulfide-Indole Motility Tubes, hydrogen sulfide production indicated by

Answer 26

blackening of the medium

Question 27

Simmons Citrate Tube differentiates bacteria according to

Answer 27

their use of citrate

Question 28

With Simmons Citrate Tube, if the bacteria use the citrate in the medium, a deep _ color develops

Answer 28

blue

Question 29

Triple Sugar Iron Agar is used for

Answer 29

presumptive ID of salmonellae and initial differentiation of enteric bacteria

Question 30

Brain-Heart Infusion broth

Answer 30

General purpose broth used to increase the number of organisms before they are plated on a solid medium

Question 31

Mannitol Salt Agar is a highly selective medium for

Answer 31

Staphylococci

Question 32

Bismuth Sulfite Agar is a _ medium and permits the growth of _

Answer 32

selective; salmonellae

Question 33

Mueller-Hinton is a _ medium

Answer 33

general purpose

Question 34

Sabouraud Dextrose and Bismuth-Glucose-Glycine-Yeast media are used specifically for

Answer 34

the culture of fungi and yeasts

Question 35

Dermatophyte Test media turns _ if dermatophytes are present

Answer 35

red