Chapter 41-Culture Techniques

Question 1

Blood agar grows

Answer 1

both gram positive and gram negative

Question 2

MacConkey agar grows

Answer 2

gram negative, gram positive do not grow

Question 3

Quadrant streaking method is designed to aid in

Answer 3

isolation of pure bacterial colonies for further testing

Question 4

In the Quadrant streaking method, isolated colonies usually grow in quadrant

Answer 4

D or 4

Question 5

In the Quadrant streaking method, streak lines should NOT

Answer 5

overlap

Question 6

How should you inoculate slants

Answer 6

butt is strapped with tip of inoculating wire and carefully withdrawn up same insertion path. Surface is then streaked in S pattern. Tube cap should be closed loosely

Question 7

For most pathogens that can invade internal organs of animals, incubate at

Answer 7

37 degrees Celsius

Question 8

Some fish, skin pathogens, and environmental organisms incubate

Answer 8

lower than 37 degrees Celsius

Question 9

Incubation time of cultures depends on

Answer 9

generation time of the species and type of medium

Question 10

Incubate plates _ to prevent condensation on the surface of the plate

Answer 10

inverted/upside down

Question 11

Method of growth for pathogens that require carbon dioxide for growth

Answer 11

candle jar

Question 12

What does the candle jar method do?

Answer 12

candle dies, leaving a decreased amount of oxygen and an increase in CO2 of the jar

Question 13

Colony characteristics

Answer 13

Size, Density, Elevation, Form, Texture, Odor, Any hemolysis

Question 14

When doing a culture of anaerobes, you should not use _ for collection

Answer 14

swabs