Chapter 43- Assisting in Blood Collection Flashcards
Phlebotomy is performed primarily to-
- aid in diagnosing disease
- monitor a patient’s condition, treatment, or medication levels
- document the existing good health of a patient
Blood borne pathogen risks are-
- Hepatitis B Virus (HBV)
- Hepatitis C Virus (HCV)
- Human Immunodeficiency Virus (HIV)
What’s the most common method to obtain a blood specimen?
Venipuncture
POL-
Physical Office Laboratory
Patient history of syncope:
- have the pt lying on an exam table or in a reclining chair
PPE-
- all facilities must stock only latex-free supplies because of the potential of allergic responders in workers and patients
- gloves
- disposable fluid- impenetrable lab coats
- protective eyewear
- face shields
Tourniquets-
- most venipuncture are done in the antecubital region
- applied 3 to 4 inches above the elbow
Antiseptics-
- the venipuncture site must be cleanses to prevent infection.
- 70 % isopropyl alcohol (rubbing alcohol)
- alcohol does not sterilize the skin but it kills many existing bacteria.
Most common collection system:
The evacuated tube system
Colored stoppers:
Indicate the tubes contents
Hemogard tops:
no splattering of blood when removed from the tube
Which tube dosen’t have any addictives?
Plain Red Topped
EDTA- Ethylenediaminetetraacetic Acid
Anticoagulant
Serum:
if blood clots and is centrifuged, the liquid portion only.
Order of the Draw:
-Blood culture bottles
– Light blue top
– Red top or gold top
– Green top
– Lavender top
– Gray top
Sterile - Blood Cultures:
Filled first because they are sterile and should not be contaminated by other tubes.
Light Blue Tube:
Sodium Citrate
Red or Gold Tube:
red serum tubes have NO anticoagulants. Gold tubes have clot activator in them and are filled next.
Green Tube:
They have plasma in their anticoagulated specimen and are used when STAT results are needed.
Lavender Tube:
they contain EDTA anticoagulant that preserves blood cell morphology. Prevents blood from clumping.
Gray Tube:
Draw last, the blood is used to test glucose or blood alcohol levels. Its additives may evaluate electrolyte levels and damage cells if passed into other tubes.
Routine Venipuncture:
Prep, Prepare, Perform, Complete
Needle Sizes:
20, 16, 23
20 Gauge:
most used in adult venipuncture.
16 Gauge:
most used by blood bank for donations
23 Gauge:
most used in infants and the elderly.
Angle:
the needle should enter the arm/vein at 15 angle.
Lumen:
Bore, or hollow space,
inside the needle; also known as
the gauge
When are syringes used?
Syringes are used when there is
concern that the strong vacuum
in a stoppered vacuum tube
might collapse the vein
Butterfly assembly used:
on small veins
What is the most common needle size?
The most common butterfly
needle size is 22 to 23 gauge with
a needle length of ½ to ¾ inch
Examples of needle safety devices:
- One-handed vacuum tube needle
– Syringe needle safety devices
– Butterfly needle safety lock
– Push-button butterfly safety device
– Needle-blunting butterfly set
What does requisitions require?
- The patient’s name, date of birth, and ID number
– The name of provider submitting the order
– The type of test required
– Test status - Patients should be asked to state and spell
their first and last name and state their birth
date - Briefly explain the venipuncture procedure
and ask them questions; answer concerns
Preparing for the Venipuncture:
- Ask the patient to extend an
arm and position his or her
other hand under the elbow to
help straighten the elbow, if
necessary - Veins in the center of the
antecubital area should be
located as a first choice - When choosing the best
available vein, palpate the
area - The tourniquet should be
placed about 3 to 4 inches
above the patient’s elbow
Performing for the Venipuncture:
-Ask the patient to extend an
arm and position his or her
other hand under the elbow to
help straighten the elbow, if
necessary
* Veins in the center of the
antecubital area should be
located as a first choice
* When choosing the best
available vein, palpate the
area
* The tourniquet should be
placed about 3 to 4 inches
above the patient’s elbow
Performing the Venipuncture:
- When you have located a vein,
remove the tourniquet - The tourniquet can remain in
place for 1 minute - Do not touch the area after
cleaning with alcohol - Anchor the vein by gently
stretching the skin downward
below the collection site with the
thumb of the non-dominant hand - The needle bevel should be facing
up - Push the evacuated tube onto
the double-pointed needle or pull
back on the syringe plunger with
the non-dominant hand
Completing the Venipuncture:
-As you remove each tube from the needle holder,
gently invert it several times before placing it in a
collection rack
* Tubes with a clot activator should be inverted 5 times
* Make sure the label contains:
– The patient’s last name, then first name
– The patient’s date of birth
– The patient’s ID number or medical insurance number
– The date and time of the draw; the phlebotomist’s initials
– The provider’s name
- If the pt was fasting
Problems Associated with Venipuncture:
Hematoma, Nerve Damage and Other Complications, Fainting,
What color tubes are drawn last?
Lavender, Green, Gray, Light Blue
Gray
Which color is the tube and stopper that is used to collect specimens for serum analysis?
Red, Green, Lavender, Blue
Red
A gray-top tube is collected for glucose testing but does not contain the required volume of blood. What effect will this have on the result?
False
Which needle lumen is used for blood bank procedures?
16 g
What effect will leaving the tourniquet on “too long” have when performing phlebotomy?
Hemoconcentration
If more than one tube must be drawn during a venipuncture, which tube is drawn first?
Blood Culture Tubes
Which location is the best site to use for capillary puncture on an infant?
Heel
Which gauge needle is used most frequently when drawing blood from the antecubital area?
21
After collecting a blood specimen for serum testing, how long must the sample sit in the rack before it is spun in the centrifuge?
30- 60 minutes
When drawing a CBC, INR, and glucose, which is the correct order of draw?
Blue, Lavender, Gray
Evacuated blood tubes, which are used to collect the patient’s blood, have color-coded tops. What does the color indicate?
Contents of the tube
What is the preferred site for a skin puncture in an infant?
Heel
To perform capillary puncture, where is the usual puncture site in adults?
Middle finger
An MA is palpating the vein of a patient during venipuncture. Which is the best finger to use for palpating a vein during venipuncture?
Index finger
Which agency establishes regulations and standards that are followed by clinical laboratories?
Occupational Safety and Health Administration
Which condition indicated a hematoma?
Presence of bruises
- A hematoma is a loss of blood into tissues.
Peeling of skin-
Sunburn can cause peel of skin which will result in damage of the epithelial layer
Vein Bulges-
When a torniquet is tied to a draw of blood
Fluid accumulation:
in the vein or tissue is edema
Which is the most preferred site for skin puncture in an 11 month old infant?
Plantar Surface of the level
While performing venipuncture, a torniquet should not be tied for longer than how many minutes at a time?
1 minute
Which is the term for the point where the needle attaches to the syringe?
Hub
Which color topped vacutainer tube contains sodium polyanethol sulfonate (SPS) as an additive?
Yellow topped tube
If hematoma forms while performing venipuncture, how long should pressure be applied?
Pressure should be applied for at least 3 minutes