chapter 4 Prokaryotic and Eukaryotic Cells Flashcards
types of prokaryotes
archaea & bacteria
types of eukaryotes
fungi/ plants/ humans
what’s the difference between prokaryotes and eukaryotes
prokaryotes have no nucleus but eukaryotes do have
a typical prokaryotic cell includes
nucleoid, bacterial chromosome, ribosomes, plasma membrane, cell wall
animal only components
centrioles and intermediate filaments
plant only components
cell wall and chloroplasts
plant and animals cell components
plasma membrane, nuclear envelope, endoplasmic reticulum, golgi appratus, lysosomes, mitochondria, microtubules, microfilaments
label a specific kind of molecule
DAPI and similar dyes bind to DNA and fluoresce only when bound able to visualize all the DNA in the cell at once
visualize a specific DNA or RNA sequence?
use a complementary strand probe for a specific sequence
how can we look at specific proteins?
GFP: green fluorescent protein; antibody specific to the protein then bind a fluorophore to the antibody
how do we study cells
separate them into their component parts and investigate
grind and find
tissue cells –> homogenization –> homogenate –> centrifugation
the nucleus
storage of genetic material, DNA replication, transcription of RNA, transport of mRNAs to the cytoplasm, assembly and export of ribosomes
nuclear envelope is a
double membrane with nuclear pore that allows transportation
Rough ER
protein synthesis for membrane and secreted proteins