13 genome engineering and recombinant DNA technology Flashcards
1
Q
restriction enzymes
A
cut DNA at specific sequences
2
Q
plasmid needs
A
1/ origin of replication
2/ region with lots of restriction enzyme sites to put DNA
3
Q
expressing an eukaryotic gene in a prokaryotic cell
A
1/ need prokaryotic promoter and terminator sequence
2/ coding sequence of the gene
3/ affinity tag to purify the protein
4
Q
gratuitous inducer
A
IPTG binds the repressor like allolactose, but is not broken down by the enzymes
5
Q
NHEJ
A
DNA ends are glued back together
6
Q
HR
A
used as a template for repair ‘ error free
