Chapter 4 -Microscopes

Question 1

Name the parts of a light microscope

Answer 1

1. Focussing knob
2. Handle
3. Eyepiece Lens (10x is the most common)
4. Tube
5. Objective lenses (4x, 10x, 40x, 100x)
6. Stage
7. Clip
   8 Mirror

Question 2

Who were microscopes invented by, and when did he invent it?

Answer 2

Robert Hooke, in 1665.

Question 3

What were the magnification and resolution capabilities of Hooke’s microscopes?

Answer 3

Magnification: x30
Resolution: about 0.002mm

Question 4

Who made better quality, single-lens microscopes, and when?

Answer 4

Antonie Van Leeuwenhoek, in 1675.

Question 5

What were the capabilities of Van Leeuwenhoek’s microscopes?

Answer 5

Magnification: x270
Resolution: 0.0014mm

Question 6

What are the capabilities of modern day electron microscopes?

Answer 6

Magnification: x2,000,000
Resolution: 0.000 000 2 mm

Question 7

Measurement triangle

Answer 7

Image size/actual size x magnification (I AM)

Question 8

What steps would you take to view an object with a microscope?

Answer 8

1. Wear eye protection
2. Collect samples
3. Add a drop of water or stain to the middle of the slide.
4. Place a sample on top of the liquid.
5. Using a toothpick, slowly and carefully lower a cover slip over the sample at an angle so as to avoid creating air bubbles.
6. Examine the sample beginning at the lowest possible magnification to locate the object.
7. Air bubbles show up as thick, black outlines.

Question 9

Which dyes are used to stain cells when observing objects through a microscope?

Answer 9

Animal cells: methylene blue

Pant cells: Iodine

Question 10

1. Name the different units of measurements.

2. Convert 100mm into the above units of measurement.

Answer 10

1. mm, micrometres, nanometres, picometres.

2. 100mm = 100,000um = 100,000,000nm = 100,000,000,000pm

Question 11

When we’re electron microscopes invented?

Answer 11

In the 1930s.