CHAPTER 4 Flashcards
What are the general principles of microscopy
Wave length of radiation, magnification, resolution, and contrast
What is magnification
Apparent increase in size of an object
What is resolution
Ability to distinguish two points that are close together. The better the resolution, the better to nearby objects are distinguished from one another.
What is microscopy
Use of light or electrons to magnify objects
What are the four types of light microscopes
Bright field, dark field, phase contrast, and differential interference contrast
What are the important parts of a compound light microscope
Objective lens, one or two ocular lenses, most have condenser lens
In a compound light microscope what does the objective lens do
Light passes through specimen into objective lens
What can the transmission electron microscope(TEM) Magnify and what is the resolving power
Magnifies 10,000-100,000 X
Resolving power 10 pm
What can the scanning electron microscope(SEM) Magnify and what is the resolving power
Magnifies 1,000-10,000
Resolving power 10 nm
What are the main differences between electron and light microscopes
Light: 2,000X magnification, 200nm maximum resolution, image produced by light
Electron: 1,000,000X or more magnification,
0.5 nm maximum resolution,
Electron beam produces image
What are the three domains
Eukarya, bacteria, and archaea
What determines what domain some thing belongs in
Ribosomal nucleotide sequences
What is taxonomy
Taxonomy consist of classification nomenclature and identification of micro organisms
What are the two different types of phase microscope’s
Phase contrast microscope and differential interference contrast microscope
What does a phase contrast microscope see
Fine structures within living cells
