Chapter 3 Study Questions (Part 3) Flashcards
what is the most significant regulatory step in glycolysis? aka what happens and who does it?
de-phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate by phosphofructokinase
what is the enzyme at the most important regulatory step in glycolysis?
PFK.
how is PFK regulated?
allosteric
fructose-1,6-bisphosphate is only used…
in glycolysis, so its formation commits the original glucose to go through glycolysis
PFK activity is inhibited by?
ATP
PFK activity is stimulated by?
ADP/AMP.
why is de-phosphorylation of F-6-P to F-1,6-BP so important?
it speeds up glycolysis. so you need to speed up the rest or else you’ll have a traffic jam.
regulatory role of F-1,6-BP have on the back half of glycolysis?
makes sure the back half goes fast and there’s not a traffic jam
product of PFK is what?
fructose-1,6-biphosphate
fructose-1,6-bisphosphate is the product of what?
PFK
how does fructose-1,6-bisphosphate speed up the back half of glycolysis?
mass action. if we speed up steps 9 to 10, we deplete 9. if 9 is depleted, 8 is higher and the reaction will rush to 9, and so on.
what is pyruvate kinase? what does it do ? what step is it featured in?
“allosteric effector” enzyme
upregulates PK activity
step 10,last step of glycolysis
what enzyme has the greatest regulatory effect in glycolysis?
phosphofructokinase
what does the location of a phosphate group on glucose tell you about how glucose can be or will be used?
basically: draw this out
