Chapter 3 Amino Acids pt 2

Question 1

The column matrix in an ion-exchange chromatagraphy is a synthetic polymer (resin) containing bound charged groups & those with bound cationic groups are called _________ & those with bound anionic groups are called __________

Answer 1

Anion exchanges, Cation-exchanges

Question 2

cation-exchange chromatography

Answer 2

The solid matrix has negatively charges groups

Question 3

Size-exclusion chromatography (gel filtration)

Answer 3

Separates proteins according to size (large proteins exit the column faster than small ones) (can be used to find the size of a protein being purified)

Question 4

Affinity chromatography

Answer 4

Is based on binding affinity (its bead contains a ligand & any proton that has an affinity for the ligand will bind to the beads)

Question 5

Chromatographic methods are enhanced by the use of __________

Answer 5

HPLC (high performance ligand chromatohraphy)

Question 6

Proteins can be separated & characterized by __________

Answer 6

Electrophoresis

Question 7

Electrophoresis

Answer 7

Use the migration of charged proteins in an electric field & is a procedure that can be used to separate proteins

Question 8

What is the equation for electrophoretic mobility (M)?

Answer 8

M=z/f (z is the net charge & f is the fractional coefficient)