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Microbiology > Chapter 3 > Flashcards

Chapter 3 Flashcards

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Biology 101 flashcards
1
Q

When using a microscope, the ability to show detail on the specimen is called the ____ _____ of the microscope.

A

Resolving power

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2
Q

What Lens of the microscope forms the magnified real image?

Objective lens
ocular lens
binomial lens
refractory light

A

The objective lens

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3
Q

_____ power × ____ power = total magnification

A

objective; ocular

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4
Q

In order to get the best resolution possible with a light microscope, you would want:
A. Long wavelengths of light
B. Short wavelengths of light
C. The wavelength doesn’t affect resolution

A

B. Short wavelengths of light

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5
Q

most widely used; specimen is darker than
surrounding field; used for live, unstained, and preserved,
stained specimens

A

Bright-field

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6
Q

brightly illuminated specimens surrounded by
______; used for live and unstained specimens

A

Dark-field

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7
Q

transforms subtle changes in light waves
passing through the specimen into differences in light
intensity, best for observing intracellular structures

A

Phase-contrast

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8
Q

Modified microscope with an ultraviolet radiation source and
filter.
Uses dyes that emit visible light when bombarded with
shorter UV rays. What kind of microscope is this?

A

Fluorescence Microscope

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9
Q

Uses a laser beam of light to scan the specimen.
Integrates images to allow focus on multiple depths or planes. This is Called a ____ microscope

A

Scanning Confocal Microscope

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10
Q

A powerful microscope used to observe the most minute specimens

A

Electron Microscopy

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11
Q

When using an electron microscope, Specimen requires special stains
or treatment.

True or false

A

true

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12
Q

When using an electron microscope Specimen is placed on___ ____ and when using a light microscope specimen is placed on ___ ___.

A

Copper mesh;Glass slide

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13
Q

What are the names of the 2 Types of Electron Microscopes ?

A

Transmit electron microscope (TEM) and
Scanning electron microscope (SEM)

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14
Q

____ ____ or ____ ____ ___allow examination
of characteristics of live cells: size, motility, shape, and arrangement

A

wet mounts; hanging drop mounts

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15
Q

____ ____ are made by drying and heating a film of specimen. This smear is stained using dyes to permit visualization of cells or cell parts.

A

Fixed mounts

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16
Q

What dye is cationic, positively charged chromophore?

A

Basic dyes

17
Q

surfaces of microbes are negatively
charged and attract basic dyes. What kind of staining is this?

A

Positive staining

18
Q

anionic, negatively charged chromophore is a ____ dye

A

acidic dye

19
Q

when using a ____ stain, the microbe repels dye and the dye stains the background

A

Negative stain

20
Q

A stain that uses only one dye and reveals shape, size, and
arrangement

A

Simple stains

21
Q

which type of stain uses a primary stain and a counterstain to distinguish cell types or parts? (examples: Gram stain, acid-
fast stain, and endospore stain)

A

Differential stains

22
Q

A stain that reveals certain cell parts not revealed by conventional methods.

A

Structural stains

23
Q

The 6 I’s of Culturing Microbes are….

A

Inoculation
Isolation
Incubation
Inspection
Information gathering
Identification

24
Q

Media can be classified according to three properties which are…

A
  • Physical state
  • Chemical composition
  • Functional type
25
Q

solid media containing beef extract,
peptone, and agar

A

Nutrient agar

26
Q

contains pure organic and inorganic compounds
in an exact chemical formula

A

Synthetic

27
Q

contains at least one ingredient
that is not chemically definable

A

Complex or nonsynthetic

28
Q

contains complex organic substances such
as blood, serum, hemoglobin, or special growth factors
required by fastidious microbes

A

Enriched media

29
Q

contains one or more ingredients that inhibit
growth of some microbes and encourage growth of the desired
microbes

A

Selective media

30
Q

allows growth of several types of microbes
and produces visible differences among those microbes

A

Differential media

31
Q

CHROMagar contains several
dyes and is used to diagnose
Urinary Tract Infections. The
patient’s sample is inoculated
and based on the color of the
colonies you can identify the
pathogen. CHROMagar is best
described as:
A. Enriched
B. Selective
C. Differential
D. Complex

A

C. Differential

32
Q

contains sugars that can be fermented, converted to acids, and a pH indicator to
show this reaction. This is known as a _____ ______ medium

A

Carbohydrate fermentation medium

Microbiology (11 decks)

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