Chapter 28 & 29 Flashcards
Bacteria
peptidoglycan in cell walls
Archaea
phospholipids in their plasma membranes
Enrichment cultures
- Growing a culture to a large enough size that you can study them
- Uses specific temperatures, substrate, nutrients and lightings
- Focuses on growing a type of bacteria and archaea
- Historically used the most
- Can be used to test theories or isolate types
- Used to test theory that bacteria could live beneath the surface of the earth
- Null prediction was that magnetite would not grow
- Magnetite did grow so one of the bacteria produced it and can live below the earth’s surface (860-2800 m)
Direct sequencing
Used when the bacteria or archaea can’t be grown in cultures
Isolates genes and determines if they are different from know gene databases
Direct sequencing lead to archaea
Use polymerase chain reaction to purify the DNA
Insert genes into plasmids
Grow a culture
Purify genes again
Sequence the genes
This method destroyed our understanding of extermeophiles
Molecular Phylogenies
Placing bacteria or archaea on trees allows scientists to understand the relationships of these small organisms
The trees are still being worked on
RNA
Biological impact
Ancient abundant and diverse
Oldest known fossil 3.5 billion years old bacteria
1.7 billion years prokaryotes ruled the earth
400 species = small intestine
128 species = stomach lining
500 species= mouth (200 unnamed or described)
Abundance
10^12 microbes on your skin and 10^ 14 in you stomach and intestines
10^3 cells make up your body
1 ml of seawater = 10,000-100,000 microbes
10% of the worlds mass of living material=dead sea microbes
Medical importance
Pathogenic bacteria are a fraction of the bacteria that live on your body, but they attack your bodies normal functions
There are several different lineages in the bacteria tree
Koch’s postulates
- Koch determined the link between diseases and bacteria
- Four postulates
- Microbe present in sick people only
- Microbe isolated and grown in a culture
- If the culture is put into a healthy organism they should get sick
- The microbe should be isolated again
Germ Theory
Koch’s postulates-> Germ theory
States that diseases are infectious
sanitation
What makes bacteria pathogenic?
Heritable trait
One pathogenic strain
The microbes then latch onto host cells and secrete toxins
Can cause different symptoms
Diarrhea or other expulsions from the body then leads to the microbes being spread even more if sanitation is poor
Antibiotics
can be used to target pathogenic bacteria using target sites
Amoxicillian
1949’s Penicillin
Since then antibiotic resistant strains
Amoxicillian
with a broad spectrum of bactericidal activity against many Gram-positive and Gram-negative microorganisms
MRSA (Methicillin-resistant Staphylococcus aureus)
vancomycin
Bioremediation
Fertilization to encourage natural bacteria and archaea growth
Seeding to make an area healthier
To gather oil or other pollutants
Example gulf oil spill
Extremophiles
- Has helped make sense of the tree of life
- Unique in the habiatats they live in
- pH less that 1.0
- Depths past 2500 M
- Anoxic environment
- 121 degrees celcius (highest)
- Live in areas that we believe are where life started
- Use extremophiles to model what possible alien organisms look like
- Commercial applications
Morphological diversity (Bacteria only)
Size: .15 micrometers^3 to 200X 10^6 micrometers
Shapes :filaments, chains, spirals
Motility: flagella and gliding (mechanism unknown)
Cell wall composition (bacteria)
Gram positive and negative
Gram positive
plasma membrane, peptidoglycan and a cell wall
penicillin, because it disrupts peptidoglycan
Gram negative
plasma membrane cell wall, phospholipid bilayer and peptidoglycan
erythromycin, poising the bacterial ribosomes
Cell walls
Knowing this allows us to treat the cells differently
Metabolic diversity
Three ways of ATP synthesis
Phototrophs
Chemoorganotrophs
Chemolithotrophs
Phototrophs
photophosploration and light
Chemoorganotrophs
oxidize organic molecules, cellular respiration or fermentation
Chemolithotrophs
oxidize inorganic molecules, cellular respiration
Photophosphorlation
Bacteria and archaea don’t have to use water as an electron receptor
Typically H2S
They can also complete anoxygenic photosynthesis (without or limited in oxygen)
Fermentation
Less efficiant
Typically a second option (not for bacteria and archaea)
Can use molecules other than glucose and can make molecules other than ethanol and lactic acid
Exp: rotting flesh smell is a by product of fermentation called cadaverine and putrescine
Cellular respiration
Requires: organic compounds, oxygen and water
Bacteria and Archaea do not follow these rules
Carbon synthesis
Two ways of getting building blocks
Autotrophs
Heterotrophs
Oxygen
21% of the atmospheric molecules
When the planet was formed there was none
Cyanobacteria: 1st photosynthetic bacteria
Nitrogen Fixation
Typically in cyano bcteria and in bacteria near plants
Nitrogen was a limiting factor
The evolution of the enzymes to fix nitrogen make the nitrogen usable
Nitrogen warning
Using nitrogen as a fertilizer is great until it reaches the water and causes mass cellular death due to blooms
Bacterial Lineages
will focus on six
- Firmicutes
- Spirochetes
- Actinobacteria
- Chlamydiae
- Cyanobacteria
- Proteobacteria
Firmicutes
- Low Guanine and Cytosine
- Gram positives
- Rod or spherical
- Chains or tetrads
- 1100 species
- Variety of ATP formations
- In the human gut
- Anthrax, botulism, tetanus, gangrene
- Insecticide,
- Yogurt and cheese fermentation
- decomposition
Spirochetes
Corkscrew shape and flagella
Outer sheath
Fermentation is the most common
Syphilis, Lyme,
Many live in anaerobic conditions
Actinobacteria
- High guanine and cytosine
- Gram positive
- Rods or filament
- Mycelia: long chains of filaments in soil
- Most heterotrophs some parasitic
- 500 antibiotics from Streptomyces alone
- Tuberculosis and leprosy
- Swiss cheese
- Break down of herbicides, nicotine and caffeine