Chapter 15 - The Genetic Code & Translation Flashcards
Which of the following is considered a secondary structure of proteins? A. Hairpin loops B. Helix-turn-helix domains C. Stem-loops D. Alpha helix E. Zinc fingers
D. Alpha helix
If instead of 20 amino acids there were 200 amino acids, what would you predict would be the minimum number of bases per codon?
4 bases per codon
4^4 = 256 (base = possible nucleotides; exponent = number of bases per codon)
AGG (tRNA) / UCC (mRNA): What is the first nucleotide at the 5’end of the anticodon if mRNA is 5’ - 3’?
G
Any given mRNA sequence has __ possible reading frames, and the correct one is determined by a(n) __ codon.
Any given DNA sequence has __ possible reading frames.
3; start / initiation codon (almost always AUG)
6 (3 per strand until the template strand is determined).
Suppose you have identified a segment of genomic DNA that contains the YFG locus.
How many possible reading frames are there for the ORF of the mRNA?
Six.
Initially, you do not know which strand is the sense strand for the YFG gene. Each strand has 3 possible reading frames. Therefore, there are 6 possible reading frames until the sense strand is established.
Poly(U) is translated into a polypeptide consisting of all phenylalanine. Poly(C) is translated into a polypeptide consisting of all proline. When a random co-polymer of U and C is made, a protein consisting of the four amino acids—leucine, phenylalanine, proline, and serine—is made even though there are eight possible codons. From this finding, it is possible to conclude that:
A. some of these four amino acids are specified by more than one codon.
B. the genetic code must be a triplet code.
C. there is only one codon each for phenylalanine and proline.
D. there are three different codons for both serine and leucine.
A. some of these four amino acids are specified by more than one codon.
Might be D, but you can’t assert that without proof!
Which statement is true regarding the binding of an amino acid to a tRNA?
A. Amino acids bind to the 5’ end of the tRNA.
B. The binding of amino acids to tRNAs requires GTP.
C. There is one aminoacyl tRNA synthetase for each of the 20 amino acids.
D. Each aminoacyl tRNA synthetase recognizes a different tRNA.
C. There is one aminoacyl tRNA synthetase for each of the 20 amino acids.
The one gene, one enzyme hypothesis evolved to the one gene, one ___; then to one gene, two or more related polypeptides called ___ or ___.
polypeptide; isoforms or variants
What roles to proteins fill? (CREDS)
communication; regulation; enzymes, defense (immunity); structure
All amino acids have 4 parts to their basic structure: a ___ _ atom, a ___ atom, an ___ group; a ___ group; and an _ group
central C; hydrogen; amino; carboxyl; R
There are __ major categories/groups of amino acids; therefore, if one is switched within the __ group, the overall effect will likely be __. Exceptions include __ (because they’re rare and designate the starting point); __ (due to disulfide bonds that are critical to proteins and their structure); and __ (they often cause bends in polypeptides).
5; same; minimal
Met (methionine); Cys (cysteine); Pro (proline)
__ (an AA) is fairly rare, often plays a role in __ activity, is one of 2 AA’s with a _ atom; has only one codon which is the ‘start’ codon (___).
Met (M); enzymatic; sulphur; AUG
T or F: R groups with aromatic rings are hydrophilic
false; benzene and other hydrocarbon rings (think crude oil) is hydrophobic
__ is one of two AAs that have a __ atom, and are important because they form __ bonds.
Cys (C); sulphur; disulfide
__ is an amino acid that often leads to bends in a __. In addition, it has no free __ group.
Pro; polypeptide
amino
These two AAs are prominent in histones.
Lys (K); Arg (R)