Chapter 13 - Analysis of Organic Compounds by Chromatography

Question 1

chromatography

Answer 1

technique used for separating the components of a mixture.

involves mobile phase with carries mixture to be separated over stationary phase

Question 2

chromatogram

Answer 2

pattern of bands or spots formed

Question 3

Rf value equation

Answer 3

distance the component travels from the origin / distance the solvent front travels from the origin

Question 4

thin layer chromatography

Answer 4

uses a thin layer of fine powder spread on a glass or plastic plate as the stationary phase

Question 5

two way chromatography

Answer 5

used to seperate substances with similar Rf values

Question 6

advantages of paper chromatography

Answer 6

cheap.
requires little preparation.
more efficient for polar and water soluble compounds.
easy to handle and store

Question 7

advantages of thin layer chromatography

Answer 7

detects smaller amounts.
achieves better separation of less polar compounds.
can use corrosive materials.
wide range of stationary phases

Question 8

eluent

Answer 8

solvent and separated component as it leaves the column

Question 9

calibration curve

Answer 9

graph plotted from measured peak areas for each component versus concentration of each component