Chapter 12 Flashcards
genetic engineering
the direct manipulation of genes for practical purposes
genetically modified organism(GMO)
an organism that has acquired one or more genes by artificial means. If the gene is from another organism, typically another species, the recombinant organism is also known as a transgenic organism
genomic library
the entire collection of DNA segments from an organism’s genome. Each segment is usually carried by a plasmid or phage.
genomics
the study of whole sets of genesand their interactions
human gene therapy
a recombinant DNA procedure intended to treat disease by altering an afflicted person’s genes
vaccine
a harmless variant or derivative of a pathogen, used to stimulate a host organism’s immune system to mount a long term defense against the pathogen
Biotechnology
The manipulation of living organisms to perform useful tasks. Today it involves DNA technology.
Clone
As in a verb to produce genetically identical copies of a cell, organisms or DNA molecule. As in noun, the collection of cells, organisms, or molecules resulting from cloning; also, a single organism that is genetically identical to another because it arose from the cloning of a somatic cell.
DNA Ligase
An enzyme, essential for DNA replication, that catalyzes the covalent bonding if adjacent nucleotides.
DNA Profiling
A procedure that analyzes an individual’s unique collection of genetic markers using PCR and gel electrophoresis. DNA profiling can be used to determine whether two samples of genetic material were derived from the same individual.
DNA Technology
Methods used to study or manipulate genetic material.
Forensics
The scientific analysis of evidence for crime scene investigations and other legal proceedings.
Gel Electrophoresiscs
A technique used for sorting macromolecules. A mixture of molecules is placed on a gel between a positively charged electrode and a negatively charged electrode; negative charges on the molecules are attracted to the positive electrode and the molecules migrate toward that electrode. The molecules separate in the gell according to their rates of migration.
Gene Cloning
The production of multiple copies of a gene.
Transgenic Organism
An organism that contains genes from another organism, typically of another species.