Chapter 11 - DNA Replication Flashcards

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1
Q

What do we call the two new made DNA strands?

A

We call them daughter strands

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2
Q

What are the three different models of DNA replication?

A
  1. conservative model
  2. semiconservative model
  3. dispersive model.
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3
Q

What is the conservative model?

A

Both the parental strands stay together after DNA replication.

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4
Q

What is the semiconservative model?

A

The double stranded DNA contain one parental strand and one daughter strand.

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5
Q

What is the dispersive model?

A

Parental and daughter strands are interspersed in both strands following the DNA replication.

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6
Q

Who are the scientist that were able to distinguish between the parental and the daughter strands?

A

Mathew Mealson and Franklin stahl.

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7
Q

Where does the DNA synthesis begin?

A

It begin at the origin of replication.

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8
Q

What is the origin of replication for E coli?

A

It is oriC

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9
Q

What are the three important DNA sequence in oriC?

A
  1. DNA boxes: It is the site where DNA A protein bind
  2. A-T rich region: sites where the DNA strands separate
  3. GATC methylation sites: sites that help regulate DNA replication
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10
Q

What is the functionality of DNA a protein?

A

It helps with addition to other proteins to bend the DNA molecule. It causes the region to wrap around the DNA a protein and separate the A-T rich region.

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11
Q

What is the function of DNA helicase?

A

It separate he DNA strands in both direction. ( breaks the hydrogen bonds between strands)

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12
Q

What is the function of Topoisomerase II ( DNA gyrase)?

A

It alleviates positive supercoiling.

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13
Q

What is the function of single stranded binding protein?

A

It keeps the parental strands apart.

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14
Q

What is the function of primase?

A

It synthesizes an RNA primer.

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15
Q

What is the function of DNA polymerase III?

A

It synthesizes a daughter strand of DNA.

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16
Q

What is the function of DNA polymerase?

A

It excises the RNA primers and fill in with DNA..

17
Q

What is the function of DNA ligase?

A

It covalently links the backbone of DNA in the okazaki fragments.

18
Q

What is DNA polymerase III composed of?

A

It is composed of 10 different subunits.

19
Q

What is the function of alpha Subunit?

A

It catalyzed bond formation between adjacent nucleotides.

20
Q

What is DNA polymerase I composed of?

A

It is composed of single polypeptide.

21
Q

What is the function of Beta subunit?

A

It holds the DNA polymerase III from falling off the template.

22
Q

What is exonuclease?

A

It digest the newly synthesized strand until the mismatched nucleotide is removed. it works from 3 to 5.

23
Q

Where are the termination sequence located?

A

They are located at the opposite side of oriC

24
Q

What happen when Tus bind to Ter?

A

It stops the movement of the replication fork.

25
Q

Where does the Eukaryotic replication begin?

A

It begins at the pre replication complex (preRC)

26
Q

What includes in the preRC ?

A
  1. ORC, origin recognition complex

2. MCM helicase

27
Q

What does the term telomere refers to?

A

It refers to the telomeric DNA complex sequence and the bound proteins.

28
Q

What does the telemetric sequence consists of?

A
  1. several guanine nucleotides

2. many thymine nucleotides.