Chapter 07: DNA Structure and Replication Flashcards
How many hydrogen bonds are between adenine and thymine?
Two
How many hydrogen bonds are between cytosine and guanine?
Three
What bonds nucleotides on the same strand?
Phosphodiester bonds
What bonds connect nucleotides across strands?
Hydrogen bonds
What is the role of topoisomerase?
Relaxes supercoiled DNA by cutting, rotating, and rejoining supercoiled areas
What is the role of helicase?
Breaks hydrogen bonds to unwind DNA strands
What is another name for helicase?
DnaB protein
What is the role of single-strand binding proteins?
Coats single-stranded DNA to prevent reannealing
What is the role of primase?
Synthesizes RNA primers on leading and lagging strands
What is the role of DNA polymerase III?
Synthesizes DNA 5’ to 3’ continuously along the leading strand and discontinuously along the lagging strand
What is the role of DNA polymerase I?
Removes nucleotides of RNA primers, replacing them with DNA nucleotides
What is the role of DNA ligase?
Catalyzes phosphodiester bonds to join DNA segments/Okazaki fragments