CH10&11 Flashcards
CO2 principle carbon source (in air)
autotrophs
CO2 from reduced/pre-formed organic molecules (other living things)
heterotrophs
carbon source trophs
autotrophs and heterotrophs
energy source trophs
phototrophs and chemotrophs
energy source from light
phototrophs
energy source from oxidizing organic/inorganic compounds
chemotrophs
ex: us
electron/H+ source trophs
lithotrophs and organotrophs
electron/H+ source from reduced inorganic compounds
lithotrophs
electron/H+ source from organic compounds
organotrophs
example of photolitoautotroph
photosynthetic bacteria, algae, plants
example of photoorganoheterotroph
purple and green non-sulfur bacteria
example of chemolithoautotroph
iron, sulfur, hydrogen, and nitrifying bacteria
example of chemoorganoheterotroph
protozoans, fungi, animals
aerobic respiration equation with donor and acceptors
NAD(P)+ + H+ —–> NAD(P)H (donnor)
or
FAD + 2H+ + 2e- ——> FADH2 (donnor)
1/2 O2 (acceptor) + 2H+ + 2e- —-> H2O
anaerobic respiration equation with donor and acceptors
NAD(P)+ + H+ —–> NAD(P)H (donnor)
or
FAD + 2H+ + 2e- ——> FADH2 (donnor)
sulfate SO4^2- (acceptor)
or
NO3- (acceptor) + 2H+ +2e- ——-> NO2- + H2O
fermenation (anaerobic respiration) equation with donor and acceptors
NAD(P)+ + H+ —–> NAD(P)H (donnor)
Acetaldehyde (acceptor) + 2H+ + 2e- —–> ethanol
or
Pyruvate (acceptor) + 2H+ + 2e- —–> lactate^2-
chemolithotrophy equation with donor and acceptors
NO^3- + 2H+ +2e- ——-> NO2- (donnor) + H2O
or
NO^2- + 8H+ +6e- ——-> NH4+ (donnor) + 2H2O
1/2 O2 (acceptor) + 2H+ + 2e- —-> H2O
series of sequential oxidation/reduction rxns
electron transport chain
location of electron transport chain in eukaryotes and prokaryotes
eukaryotes- mitochondria
prokaryotes- plasma membrane
terminal electron acceptors in aerobic and anaerobic
aerobic: O2
anaerobic: nitrate, sulfate, archaea use CO2
energy from the electron transport chain oxidation generates a proton motive force from
H+ [ ] gradient/ charge difference across membrane
outside vs inside charge difference in electron transport chain
outside: H+
inside: -
what does the ETC allow
ATP synthase of protons back inside across membrane
How does the ETC work in bacteria
- electron donnors give off 2 e- tht go into the
ETC
-H+ are pumped by ETC from the inside to the outside of the plasma membrane
-the 2 e- are passed thru the ETC and given to the electron acceptor
-the proton motive force allows the H+ to get pumped back thru membrane and thru ATPase that converts ADP + Pi —-> ATP + H2O
in bacteria the proton motive force (PMF) is used for:
- synthesis of ATP- protons move thru ATPase
- motility- protons move thru channel in basal of flagella causing it to spin
- active transport- uptake of nutrients against a [ ] gradient
describe gylcolysis
- as glucose to being converted to 2 pyruvate:
- 2 ATP are converted to 4 ATP (2 generated)
- substrate level phosphorylation
- 2NAD+ to reduced to 2NADH
- the 2NADH go into ETC produces 5 ATP (oxidative phosphorylation) and 1/2 O2 is converted to 2 H2O in ETC
- 2 ATP are converted to 4 ATP (2 generated)
7 ATP generated
describe pyruvate oxidation
2 pyruvate produce 2 Acetyl-CoA
5 ATP produced (oxidative phosphorylation)
describe the TCA cycle
oxidative phosphorylation (NADH) 15ATP oxidative phosphorylation (FADH) 3 substrate level phosphorylation (GTP) 2
total 20
total aerobic ATP yield
32 ATP
fermentation donnor and acceptor
donnor-FADH
acceptor- acetaldehyde, pyruvate
- generate NADH+
- low energy yield (substrate level phosphorylation, no ETC or PMF)
- toxic end product
fermentation
of ATPs produced in fermentation
2 ATP
what happens if no O2 in fermentation
glycolysis backs up from the back up of NAD+
nitrifying bacteria
Nitrosomonas or Nitrobacter
what is NH4+ and 1 1/2 O2 in Nitrosomonas sp. reduced to
NO2 and H2O
what is NO2- in Nitrobacter sp. reduced to
NO3-
importance of terrestrial habitats for nitrifying bacteria
- plants use of NO2-
- plants don’t take up as quickly
why is it bad if plants cannot take up all the NO2- in soil and it seeps into water sources?
- it may seep into streams, algae can grow from it
- once algae dies, bacteria oxidize the algae causing fish to die from the lack of O2 in the water
-infants/children cannot drink water with Nitrite bc it can cause blue baby syndrome bc Nitrate binds to hemoglobin
why wont NH4+ wash out of soil?
bc the soil is -, so it will hold the + NH4+
most to least energy yield with their e- carries
most -aerobic- O2 -anaerobic- NO3-, SO4^2-, CO2 -chemolithotrophy- O2, SO4^2-, NO3- -fermentation- pyruvate acid least
sulfur oxidizing bacteria
Acidothiobacillus ferrooxidan
acid mine drainage is from the bacteria called
Acidothiobacillus ferrooxidan
advantage of acid mine drainage
used for bio mining- slow process
- reduced inorganic compounds are e- donors
- O2 is usually acceptor
- prokaryotic
- low energy yield- oxidize large quantities of nutrients
chemolithotrophy
organic electron donors for chemoorganotrophy
-aerobic- O2 \+++ ETC -anaerobic- NO3-, SO4^2-, CO2 \++ ETC -fermentation- pyruvate acid (endogenous organic e- acceptor) \+ ETC
inorganic electron donors for chemoorganotrophy
-chemolithotrophy- O2, SO4^2-, NO3-
+ ETC