CH1 Flashcards
first to efficiently observe MOs and called them animalcules
Leeuwenhoek
tiny living things Leeuwenhoek saw under the microscope at 300x
animalcules
- animalcules result from fermentation/putrefaction
- chemical changes
- to occur, needs air (vital force)
abiogenesis or spontaneous generation
- animalcules come from other animalcules causes causes fermentation/putrefaction
- must arise from other living things
biogenesis
- concluded that MOs don’t spontaneously appear
- experiment with cork: vital force
Redi
- no spontaneous generation
- MOs accumulate in in bends of flask
- let air in without MOs getting in
- experiment done by Pasteur
swan neck flask experiment
said that if there is air, then there is MOs
Tyndall
- MOs produce fermentation products, not the reverse
- heat enough to kill MOs
- pasteurization
germ theory of fermentation
- 50-60 degree C (120-140 degree F)
- kill specific unwanted MOs
- doesn’t alter taste/texture
- originally for wine
pasteurization
isolated anthrax that is harmful to cattle and pasture land
-claimed one kind of MO causes one disease
Koch
caused by rod shaped bacterium with spores and introduced controls measures
anthrax
to prove the causative agent of a disease
Koch’s postulates
Name Koch’s Postulates and modifications
- specific MOs always associate with a given disease
- MO isolated and grown in pure culture in the lab
- pure culture will produce disease
- recover the injection MO from experimentally infected organism
modifications
-viruses, other obligate parasites same diseases caused by more than one MO, one MO may cause several different diseases
- causative agent of tobacco mosaic disease and would show on leaves
- something was smaller and went through filter
- filterable
Ivanovski and Beilernck (viruses)
- first human disease known that was caused by disease
- transmitted by mosquitoes
- agent filterable
- Walter Reed
yellow fever
- prevent infection
- practiced before Koch’s Germ Theory of Disease proven
antisepsis
- used to clean hands of obstreticians from giving expecting mothers childbed fever (perpetual fever)
- Semmelweis
chlorine
Lister used this to soak his surgical dressings in to prevent antisepsis in wounds
carbolic acid (phenol)
stimulation of host to develop resistance to a specific disease
immunization
- created smallpox vaccine
- inoculated James Phipps with cowpox the with smallpox, he is resistant to smallpox
Jenner
outline Pasteur’s chicken cholera experiment
- inoculated chicken with pure culture of chicken cholera bacteria 8 week old
- after several weeks, the chicken remained healthy
- Pasteur realized he used old culture and not fresh culture
- with fresh virulent culture of chicken cholera bacteria, he inoculated the chicken previously inoculated and one that was not
- previously inoculated chicken lives and the one that wasn’t dies
drugs that treat microbial diseases
chemotherapy