ch 8 Flashcards
why do cells have nucleic acids
building blocks for DNA RNA proteins, needs for replication, transcription, and translation
DNA replication
semiconservative, bidirectional, DNA poly, templates, nucleotides, primers, timed cell growth and division,
how DNA is marked
methylating a or c
restriction endonucleases
enzymes that cuts DNA that is not marked as its own
rna synthesis via
transcription,
needs promoters, sigma factors, RNA poly.
elongation, termination (rho indie and rho dependent)
operons
coordinate expression of genes that function in same bio pathway (lac), transcribed together
coupled transcription and translation
in pro, DNA can be read and translated as its being transcribed
shine dalgarno sequence
translation initiation sequence
translation steps
initiation, elongation, termination
pro ribosome vs euk
70s vs 80s
covalent modification example
translation processing
termini cleave, chem group added, single peptide removed
gyrase
relieves positive supercoils
Initiation involves
Promoter, Sigma factor, RNA Poly
sigma factor
promote binding of RNA polymerase to promoter
rho independent
forms hairpins