19 Flashcards
themes of microbes
ubiquity, abundance metabolic power, frequently in communities
syntrophy
microbes team up
is there is a theromdynamically feasible reaction, microbes will
exploit it
microbes impact
carbon cycle, nitrogen cycle, sulfur cube, phosphorus cycle, gases
enrichment culture is used to
isolate and grow rare organisms with specific characteristics from mixed populations
interdependent nutritional consortia
bacteria NEED each other to grow
why is it difficult to grow certain bacteria in lab
difficult to replicate optimal growth conditions, unknown growth conditions
why cultivate microbes
study; genome, physiology, metabolism
microbe domestication
microbe does not exhibit traits found in nature
Amplicon-based community fingerprinting example
amplify 16s ribosomal RNA to determine abundance of stuff in a sample
cultivation independant methods
FISH, amplicon based community fingerprinting, antibodies, single cell genomics, metagenomics
RNA tells
OTU (operational taxonomic units), abundance, phylotypes
phylotypes
microbes Identified by sequence similarity
genome analysis steps
shearing, sequencing, assembly, annotation
proteome analysis steps
gel based separation, isolated tryptic digest, peptide sequencing/ mass fingerprinting