19 Flashcards

1
Q

themes of microbes

A

ubiquity, abundance metabolic power, frequently in communities

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2
Q

syntrophy

A

microbes team up

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3
Q

is there is a theromdynamically feasible reaction, microbes will

A

exploit it

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3
Q

microbes impact

A

carbon cycle, nitrogen cycle, sulfur cube, phosphorus cycle, gases

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4
Q

enrichment culture is used to

A

isolate and grow rare organisms with specific characteristics from mixed populations

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5
Q

interdependent nutritional consortia

A

bacteria NEED each other to grow

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5
Q

why is it difficult to grow certain bacteria in lab

A

difficult to replicate optimal growth conditions, unknown growth conditions

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6
Q

why cultivate microbes

A

study; genome, physiology, metabolism

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7
Q

microbe domestication

A

microbe does not exhibit traits found in nature

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8
Q

Amplicon-based community fingerprinting example

A

amplify 16s ribosomal RNA to determine abundance of stuff in a sample

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9
Q

cultivation independant methods

A

FISH, amplicon based community fingerprinting, antibodies, single cell genomics, metagenomics

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10
Q

RNA tells

A

OTU (operational taxonomic units), abundance, phylotypes

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11
Q

phylotypes

A

microbes Identified by sequence similarity

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12
Q

genome analysis steps

A

shearing, sequencing, assembly, annotation

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13
Q

proteome analysis steps

A

gel based separation, isolated tryptic digest, peptide sequencing/ mass fingerprinting

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14
Q

Metabolome analysis

A

derivatization, GC-MS, identification analysis, total metabolites

15
Q

trancriptome

A

total rna, reverse that biddy