Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacteriology > Ch. 7 - Traditional Cultivation and Identification > Flashcards

Ch. 7 - Traditional Cultivation and Identification Flashcards

0
Q

2 bacteria that cannot be developed in vitro

A
  • Treponema pallidum

- Mycobacterium leprae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
1
Q

process of growing microorganisms in culture

A

Cultivation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

bacteria with complex needs and exceptional media components

A

fastidious

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Bacteria that has relatively basic and straightforward needs

A

Nonfastidious

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Indicates bacterial growth in broth media

A

Turbidity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Amount of bacteria needed for turbidity to be detected by the naked eye

A

10^6 bacteria per milliliter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Type of broth media where the location of growth indicates the type of bacteria present based on oxygen requirements

A

Thioglycollate broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Bacteria that grow at the bottom of Thioglycollate broth

A

Strict anaerobes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Bacteria that grow near the surface of Thioglycollate broth

A

Aerobic bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Type of bacteria that will grow throughout the Thioglycollate broth media

A
  • Facultative anaerobes

- Aerotolerant organisms

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Most common solidifying agent

A

Agarose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

The formed stable solid gel in solid medium

A

Agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Petri dish containing agar

A

Agar plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

The bacterial population is considered to be derived from a single bacterial cell

A

Pure colony

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Media that contains specific nutrients required for the growth of particular bacterial pathogens

Used to enhance growth of a particular bacterial pathogen by providing specific nutrients for the organism’s growth

A

Enrichment media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Enrichment broth for isolation of anaerobes

A

Thioglycollate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Enrichment media for Group B streptococci

A

LIM broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Enrichment media for enteric gram-negative organisms

A

Gram-negative (GN) broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Media that contains nutrients that support growth of most nonfastidious organisms without giving any organism a growth advantage

A

Nutritive media/Supportive media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Nutritive media for bacteria

A
  • Tryptic soy agar

- Nutrient agar plates

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Nutrient media for fungi

A

Sabouraud’s dextrose agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Media that contains one or more inhibitory agents (dyes, bile salts, alcohols, acids and antibodies)

A

Selective media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Selective media for gram-positive cocci; inhibits aerobic and facultatively anaerobic gram-negative rods

A

Phenylethyl alcohol (PEA) agar

23
Q

Media that allows colonies of one bacterial species or type to exhibit certain metabolic or culture characteristics that can be used to distinguish it from other growing bacteria

A

Differential media

24
Q

Differential media to differentiate gram-negative bacteria that can and cannot ferment the sugar lactose

A

MacConkey agar

25
Q

Most commonly used nutritive medium because it allows many organisms to grow

Also differential because appearance of colonies produced by certain bacterial species is readily distinguishable

A

Sheep blood agar

26
Q

Agar for culturing a patient’s blood for bacteria

A

Brain-Heart Infusion (BHI) agar

27
Q

Agar for cultivation of Haemophilus spp. & Neisseria gonorrhoeae

Hemoglobin, hemin and nicotinamide adenine dinucleotide (NAD) are released into the agar

A

Chocolate agar

28
Q

Agar that allows gram-positive bacterial growth

Can be used to differentiate bacterial colonies on the hemolytic reactions they produce

Contains 2 antibiotics

A

Columbia CNA agar with blood

29
Q

Antibiotic that disrupts cell membranes of gram-negative organisms

A

Colistin

30
Q

Antibiotic that blocks DNA replication in susceptible organisms

A

Nalidixic acid

31
Q

Agar for the selective growth of gastrointestinal pathogens (Salmonella spp. & Shigella spp.)

Contains mannitol

A

Gram-negative (GN) broth

32
Q

Primary source of carbon in media. Favored energy source for many enteric pathogens.

A

Mannitol

33
Q

Agar for growth of Salmonella spp. and Shigella spp.

Differential for lactose and non-lactose fermenting gram-negative bacilli: non-lactose fermenters maintain blue-green color of the agar

Contains ferric ammonium citrate for detection of H2S (black ppt) produced by Salmonella spp.

A

Hektoen Enteric (HE) agar

34
Q

Color of Salmonella spp. and Shigella spp. in Hektoen Enteric agar

A

Blue-green (original color of the medium)

35
Q

Agar for gram-negative bacilli

Differentiates lactose and non-lactose fermenters: non-lactose fermenters remain colorless(Shigella spp.)

A

MacConkey agar

36
Q

Agar for gram-positive cocci

A

Phenylethyl Alcohol (PEA) agar

37
Q

Agar that supports growth for all but the most fastidious clinically significant bacteria

A

Sheep blood agar

38
Q

Complete clearing of the RBCs around the bacterial colony

A

Beta hemolysis

39
Q

Partial lysis of RBCs to produce a greenish discoloration around the colony

A

Alpha hemolysis

40
Q

Enrichment and selective agar for the isolation Neisseria gonorrhoeae & Neisseria meningitidis

Has 3 antibiotics: Colistin, Vancomycin, Nystatin

A

Modified Thayer-Martin (MTM) agar

41
Q

Antibiotic that inhibits gram-positive bacteria

A

Vancomycin

42
Q

Antibiotic that inhibits yeast

A

Nystatin

43
Q

Antibiotic that may be added to MTM agar that inhibits Proteus spp. which may swarm over the agar surface and mask detection of individual colonies of the 2 Neiserria spp.

A

Trimethoprim

44
Q

A modification of MTM agar where ansamycin substitutes nystatin and there is a higher concentration of vancomycin

A

Martin-Lewis agar

45
Q

Enrichment broth most frequently used in diagnostic bacteriology

A

Thioglycollate broth

46
Q

Agar that is selective and differential for Shigella spp. and Salmonella spp.

Salmonella spp. often exhibits a black center due to production of H2S

A

Xylose Lysine Desoxycholate (XLD) agar

47
Q

3 bacterial pathogens that require living cells for cultivation

A
  • Chlamydiae
  • Rickettsiae
  • Ricketstiae-like organism
48
Q

Type of organisms that grow best with higher CO2 concentration

A

Capnophilic

49
Q

Primary lab devices used to provide the environmental requirements for cultivating microorganisms

A

Incubators

50
Q

Optimum temperature for incubation

A

35-37 C

51
Q

Optimal amount of CO2 for incubation

A

3-5% CO2

52
Q

Optimum pH level for incubation

A

6.5 - 7.5

53
Q

Temperature at which Campylobacter jejuni is able to grow

A

42 C

54
Q

Temperatures that enable Listeria monocytogenes and Yersinia enterocolitica to grow

A

4 - 43 C

55
Q

Type of organisms that require only low levels of oxygen

A

Microaerophilic/Microaerobic

Bacteriology (7 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions