Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BIO 1210 > Cellular Respiration & Metabolism > Flashcards

Cellular Respiration & Metabolism Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Define metabolism

A

all of an organism’s chemical reactions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is the purpose of metabolism?

A

Manages material and energy resources

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is a metabolic pathway?

A

A series of reactions to produce products, each step is catalyzed by different enzymes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are the two kinds of metabolic pathways?

A
  1. catabolic

2. anabolic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Define catabolic pathways and provide an example

A

exergonic reactions that break down of complex molecules to simpler ones

releases energy

ex. cellular respiration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

T or F: catabolic pathways are endergonic/endothermic. Why/why not?

A

FALSE.

Catabolic pathways are EXOthermic/Exergonic because they release energy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Define anabolic pathways and provide an example

A

Endergonic reactions that build complex molecules from simpler ones

Requires an energy input/consumes energy

ex. fatty acid or protein synthesis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Describe photosynthesis and provide the general chemical formula

A

A reaction that consumes energy from sunlight and converts it into chemical energy and is stored in organic compounds

6CO2 + 6H2O + light –> C6H12O6 (glucose) + O2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Define cellular respiration and provide the general formula

A

The break down of organic molecules in order to produce ATP and energy

C6H12O6 + O2 –> 6CO2 + 6H2O + energy (ATP + heat)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Describe ATP

A

Adenosine triphosphate is an energy source that powers cellular work to keep organisms alive

Function:

  • mediates most energy coupling in the cells
  • an immediate source of energy for cells
  • acts as a nucleoside triphosphate in the synthesis of RNA
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is ATP composed of?

A

a ribose (sugar), adenine (nitrogenous base), and a chain of three negatively charged phosphate groups

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What are three kinds of cellular work that ATP is involved in?

A
  1. chemical work (endothermic reactions)
  2. Transport work (active transport)
  3. mechanical work (movement of cells or cell parts)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

How are the bonds between phosphate groups in ATP broken? And what is formed when this occurs?

A

The weak bonds between phosphate groups are broken by hydrolysis in an exergonic reaction causing…
ADP (Adenosine Diphosphate) and an inorganic phosphate to form and release significant energy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What happens to the inorganic phosphate that detaches from ATP when ADP is formed?

A

It is transferred to a reactant or transport protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Cellular respiration is key in recycling which two molecules back into ATP?

A

recycling ADP and inorganic phosphate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Describe redox reactions

A

chemical reactions that involve the transfer of electrons between an oxidizing agent and a reducing agent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Which kind of reactions (exo or endo) undergo redox reactions?

A

Exothermic reactions yield energy because of the transfer of electrons (this energy is then used to synthesize ATP)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Describe reduction reactions

A

Reduction is the GAINING of electrons (element becomes MORE negative)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Describe oxidation reactions

A

Oxidation is the LOSS of electrons (element becomes LESS negative)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Describe reducing agent

A

The element/molecule that oxidizes (becomes less negative) because it has donated an electron and has reduced the other element/molecule

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Describe oxidizing agent

A

The element/molecule that is reduced (becomes more negative) because it has gained an electron by oxidizing the other molecule/element

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What is a trick to remember redox

A

OILRIG

Oxidation is LOSS

Reduction is GAIN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

In this example, which element is the reducing agent and which is the oxidizing agent:

Xe- + Y –> X + Ye-

A

X is oxidized (becomes less negative) so it is the reducing agent

Y is reduced (becomes more negative) so it is the oxidizing agent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

T or F: Oxidation can occur without oxygen being present

A

TRUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Why is oxygen such a strong oxidizing agent?

A

Because it is highly electronegative so it can easily strip electrons from other elements/molecules

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

How are redox reactions involved in cellular respiration?

A

Glucose and other food molecules are OXIDIZED (lose e-)

Oxygen (present in aerobic cellular respiration) REDUCES (gains the e-)

electrons are transferred from glucose to oxygen through a series of steps that involve redox reactions

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Why are electrons passed from glucose to oxygen through a series of steps rather than just in one step?

A

So that the harvest of energy will be more efficient. If electrons were transferred directly to oxygen in one step there would be a huge explosion and waste of energy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

T or F: each step between glucose and oxygen is catalyzed by an enzyme?

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

T or F: Electrons are transferred indirectly between glucose and oxygen along with a hydrogen proton?

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Which enzymes are involved with the transfer of electrons?

A
  • NAD+

- Dehydrogenase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

Describe NAD+

A

A coenzyme that acts as an electron carrier to transport electrons from glucose to the electron transport chain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

Describe dehydrogenase

A

An enzyme that oxidizes the substrate by removing 2e- and 2 H+ and reduces NAD+ to NADH by giving it 2e- and 1 H+

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

T or F: electrons lose a ton of energy when they are transferred from the substrate to NAD+

A

FALSE, they lose very little energy because NAD+ is a very efficient electron carrier

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

Through which mechanism do electrons travel to reach O2?

A

The Electron Transport Chain (ETC)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

What are the 4 major steps of cellular respiration? Where does each step occur?

A
  1. Glycolysis
    - occurs in the cytosol
  2. Pyruvate oxidation
    - occurs in the matrix of the mitochondria of eukaryotes
    - cytosol of prokaryotes
  3. Citric Acid Cycle (CAC)
    - occurs in the mitochondria of eukaryotes
    - cytosol of prokaryotes
  4. Oxidative phosphorylation
    - occurs in the mitochondria of eukaryotes
    - plasma membrane of prokaryotes
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

What are the two ways cellular respiration makes ATP?

A
  1. Substrate level phosphorylation

2. Oxidative phosphorylation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

Describe substrate-level phosphorylation

A

A way that cellular respiration produces ATP (although small amounts are produced)

This occurs during glycolysis and the CAC

Makes ATP by directly transferring phosphate groups from the substrate to ATP

38
Q

T or F: Substrate level phosphorylation is responsible for producing the majority of ATP in cellular respiration. Why/why not?

A

FALSE. Oxidative phosphorylation is responsible for producing the most ATP

39
Q

Describe oxidative phosphorylation

A

A way that cellular respiration produces most of the ATP

Uses the ETC and chemiosmosis to make ATP by making ADP and inorganic phosphate

40
Q

Describe glycolysis

A

The first step in cellular respiration that involves molecules of glucose being digested/broken down into molecules of pyruvate

1 molecule of glucose (6C) –> 2 molecules of pyruvate (3C each)

41
Q

In glycolysis, 1 molecule of glucose has how many carbons? And is broken down into how many of which molecules? How many carbons do the products each contain?

A

Glucose has 6 carbons

Converted into two pyruvate molecules

Each pyruvate has 3 carbons

42
Q

What are the two phases in glycolysis?

A
  1. Energy investment phase:
    - first 5 steps that invest energy and use 2 ATP
  2. energy payoff phase:
    - last 5 steps that produce 4 ATP (made by substrate level phosphorylation) and 2 NADH
43
Q

What are the inputs, outputs, and net products of glycolysis?

A

INPUTS:

  • glucose
  • 2 ATP
  • 2 NAD+, 4 e-, and 4+

OUTPUTS:

  • 2 Pyruvate
  • 4 ATP
  • 2 NADH

NET PRODUCTS:

  • 2 pyruvate, 2 H2O
  • 2 ATP
  • 2 NADH + 2 H+
44
Q

Describe pyruvate oxidation

A

if the cellular respiration will continue aerobically, pyruvate is transferred into the matrix (innermost part) of the mitochondria

Both pyruvate molecules are oxidized (lose e-) and converted into Acetyl Coenzyme A

45
Q

How many carbons does a molecule of acetyl coenzyme A have? What does this imply?

A

2 carbons per molecule of acetyl CoA

Implies that the conversion between one 3-C pyruvate molecule to a 2-C molecule of Acetyl CoA loses a molecule of CO2

46
Q

T or F: Acetyl CoA can be regenerated and used again and again for pyruvate oxidation. Why/why not?

A

True because it is an enzyme

47
Q

How many molecules of Acetyl per molecule of glucose will enter the citric acid cycle?

A

2 molecules of acetyl per molecule of glucose

48
Q

What is the reaction equation for pyruvate oxidation?

A

Pyruvate + CoA + NAD+ —> acetyl CoA + CO2 + NADH + H+

49
Q

Describe the Citric Acid Cycle

A

2 acetyl groups (contain two carbons each) per glucose molecule enter the CAC (WITHOUT CoA) to be processed through 8 steps.

Each turn of the CAC has 2 carbons enter as one acetyl group and 2 different carbons leave as oxidized CO2 (so for one glucose molecule, the cycle turns twice because 2 acetyl groups enter)

The CAC generates 1 ATP per turn by substrate level phosphorylation but most energy is transferred to NAD+ and FAD where they are reduced to NADH and FADH2 and carry electrons to the ETC

50
Q

What does each turn of the CAC produce per 1 one molecule of glucose?

A

One turn produces 2 carbons and 1 ATP molecule

51
Q

T or F: oxygen is directly required for the CAC?

A

FALSE. but it does need NAD+ and FAD for electron transport which require O2 (indirectly needs it)

52
Q

Describe the electron transport chain (ETC)

A

the ETC is a series of proteins with bound non-protein components that are essential to the catalytic function of enzymes

STEPS:
- each carrier is reduced when it accepts electrons and is oxidized as it is stripped of electrons

  • each carrier is slightly more electronegative than the previous so that the electrons can be passed to the next and move down the chain
  • NADH donates electrons to the first electron carrier in complex I
  • FADH2 adds electrons to complex II but is not as efficient so releases 2/3 as much energy as NADH (makes fewer ATP)
  • Coenzyme Q takes electrons from complexes I and II to complex III
  • Cytochrome c carries the electrons from complex III to IV
  • a cytochrome in complex IV donates electrons to O2 to make H2O
  • energy released during the ETC is coupled with ATP synthesis with the help of ATP Synthase enzymes
53
Q

Where is the ETC located and how does the structure of the mitochondria increase cellular respiration rates?

A

ETC is embedded in the inner mitochondrial membrane which is folded to form cristae

–> increase surface area so that thousands of copies of ETC can exist in one mitochondrion

54
Q

Describe oxidative phosphorylation

A

When the electrons have been transferred to O2 from the ETC, O2 makes H2O

Then the enzyme ATP Synthase makes ATP from ADP and inorganic phosphate

55
Q

Describe ATP Synthase

A

An enzyme that makes ATP from ADP and inorganic phosphate during oxidative phosphorylation

56
Q

What is the mechanism that pumps protons across the membrane by the complexes as electrons are transferred through the ETC?

A

Electrogenic pump

57
Q

Describe chemiosmosis

A

The energy coupling mechanism that uses energy stored in H+ gradient across a membrane to drive cellular work

Uses H+ gradient to power ATP synthesis
- H+ is bound to a rotor in ATP Synthase causing it to spin and activate catalytic sites

ATP is synthesized as H+ moves down their electrochemical gradient

58
Q

In summary, how many ATP molecules does NADH and FADH2 produce?

A

NADH produces 2.5 ATP
FADH2 produces 1.5 ATP

*taking into account the cost of moving ATP out of the mitochondria to cytosol for use

59
Q

What is the total amount of ATP produced by cellular respiration per molecule of glucose?

A

30-32 molecules of ATP produced per molecule of glucose

60
Q

How efficient is cellular respiration at producing ATP?

A

Only about 34% efficient, theoretically, oxidation of glucose could produce 94 ATP molecules

the rest of the energy is lost as heat

61
Q

Describe anaerobic cellular respiration

A

A catabolic process that occurs without the presence of oxygen

Instead of oxygen, the final electron acceptor is a different electronegative molecule (one less EN than oxygen)

62
Q

Describe fermentation

A

An anaerobic catabolic process that partially degrades sugars (like glucose) to produce some ATP from glucose without an ETC (no oxygen involved)

Fermentation produces a specific product like lactic acid or ethyl alcohol

63
Q

Describe lactic acid fermentation

A

Pyruvate is directly reduced by NADH to form lactate as an end product without the release of CO2

64
Q

Describe alcohol fermentation

A

Pyruvate is converted into ethanol in two steps:

  1. CO2 is released from the pyruvate which is then converted into acetaldehyde (2-C compound)
  2. Acetaldehyde is reduced by NADH to ethanol which generates the supply of NAD+ required for glycolysis to continue
65
Q

What are the 3 possible fates of pyruvate?

A
  1. in aerobic respiration, pyruvate will move into the mitochondria and be oxidized into acetyl CoA
  2. in anaerobic respiration, pyruvate will be undergo alcohol fermentation and be converted into ethanol (ethyl alcohol)
  3. Or in anaerobic respiration, pyruvate will undergo lactic acid fermentation and be converted into lactate
66
Q

What is the general pathway of carbohydrate catabolism in the cell?

A

Glycolysis can accept a wide range of carbohydrates for catabolism

  • starch is hydrolyzed into glucose which can be then be further broken down by glycolysis and the citric acid cycle
  • glycogen can also be hydrolyzed into glucose between meals to power respiration
67
Q

What is the general pathway of fat catabolism in the cell?

A

Fats are digested into glycerol and fatty acids so the glycerol can be converted into glyceraldehyde 3-phosphate (an intermediate of glycolysis)

But most of the energy of fats are stored in the fatty acids, so BETA OXIDATION breaks down the fatty acids

68
Q

Describe Beta Oxidation and which macromolecule does it act on?

A

Fats store energy mostly in their fatty acid components so for fats to be catabolized, their fatty acids are broken down by beta oxidation to be converted into 2-C fragments that can enter the CAC as Acetyl CoA

NADH and FADH2 are also produced by beta oxidation and can enter the ETC to produce more ATP

69
Q

What is the general pathway of protein catabolism in the cell?

A

Proteins are digested into their amino acid constituents and if the amino acids are in excess, enzymes convert them to intermediates of glycolysis to then enter the CAC (the amino groups are removed)

the nitrogenous remains are excreted as a form of ammonia by the animal

70
Q

What is deamination?

A

A process that removes Amino groups from amino acids during the catabolism of proteins

71
Q

Define phosphofructokinase

A

An allosteric enzyme with receptor sites for specific inhibitors and activators

72
Q

What role does phosphofructokinase play in regulating cellular respiration rate?

A

It is inhibited by ATP and activated by AMP (Adenosine Monophosphate)

When there is an excess of ATP, Phosphofructokinase is inhibited to slow down glycolysis until cellular work converts ATP back to ADP faster than ATP can be regenerated

Accumulation of citrate in the mitochondria will force citrate to move to the cytosol which will also inhibit the enzyme

73
Q

How is the rate of cellular respiration regulated?

A

With the help of the allosteric enzyme phosphofructokinase

74
Q

Which stages of cellular respiration CAN occur without oxygen?

A

Glycolysis can occur without oxygen but this will happen under anaerobic fermentation conditions

75
Q

Which stages of cellular respiration CANNOT occur without oxygen?

A

Pyruvate oxidation
CAC
Oxidative phosphorylation (ETC and chemiosmosis)

76
Q

Describe energy coupling

A

The use of an exergonic process to drive an endergonic process

77
Q

Define allosteric regulation

A

the process of stabilizing the structure of an enzyme in its active form by binding a molecule

78
Q

What purpose does allosteric regulation serve?

A

it allows cells to regulate metabolic pathways by controlling when and where various enzymes are active

79
Q

T or F: in many cases, the molecules that naturally regulate enzyme activity (allosteric regulation) behave similar to reversible noncompetitive inhibitors. why/why not?

A

TRUE because these molecules can change the shape and functioning of an enzyme’s active site by binding to a site elsewhere on the enzyme

80
Q

Define feedback inhibition

A

a metabolic pathway is stopped due to the binding of an enzyme-substrate’s end product to that enzyme

81
Q

Define cooperativity in terms of enzymes

A

a type of allosteric regulation that increases the response of enzymes to substrates

82
Q

How does hydrolysis of ATP power cellular work?

A

By releasing free energy that can be coupled to other reactions

83
Q

Define cofactors

A

a nonprotein enzyme helper, usually a metal ion

84
Q

Define coenzymes

A

A nonprotein enzyme helper, usually an organic molecule (a specific type of cofactor)

85
Q

Give an example of feedback inhibition in cellular respiration

A

ATP acts as an allosteric inhibitor to enzymes in ATP-producing pathways

86
Q

How does the active site of an enzyme lower the activation energy barrier and therefore catalyze a reaction?

A

By orientating substrates correctly to bind with the active site

87
Q

How is energy released from an ATP molecule?

A

By the hydrolysis of one of the phosphate groups to form ADP and inorganic phosphate

88
Q

T or F: Enzymes can lower the activation energy of reactions

A

TRUE

89
Q

T or F: Enzymes can change the equilibrium point of reactions

A

FALSE

90
Q

T or F: enzymes can change the net energy output of a reaction

A

FALSE

91
Q

Describe the energy relationship between anabolic and catabolic pathways

A

Anabolic pathways synthesize complex organic molecules using the energy produced by catabolic pathways

BIO 1210 (16 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions