Cellular control Flashcards
what is transcriptional level control
when gene expression is controlled by altering the rate of transcription of genes
what are transcription factors
proteins that bind to DNA and switch genes on or off by increasing or decreasing the rate of transcription
what are factors called that start transcription
activators
what are factors called that stop transcription
repressors
in prokaryotes control of gene expression often involves transcription factors binding to what
operons
what is an operon
a section of DNA that contains a cluster of structural genes that are all transcribed together as well as control elements and sometimes a regulatory gene
what do structural genes do
code for useful proteins such as enzymes
what do the controls meets contain
promoters (a DNA sequence located before the structural genes that RNA polymerase binds to and an operator (a DNA sequence that transcription factors bind to
what does the regulatory gene do
codes for an activator or repressor
how does the lac operon function when lactose is not present
regulatory gene produces lac repressor
this then binds to the operator site where no lactose is present
this blocks transcription as RNA polymerase can’t bind to the promoter
how does the lac operon function when lactose is present
lactose binds to the reposer, changing its shape so it can no longer bind to the operator site
RNA polymerase can then begin transcription in structural genes
what is the lac operon, where is it found and what does it do
where the genes are found that produce enzymes needed to respire lactose
found in E coli
what is post-transcriptional level control
when control of gene expression takes place after post-transcriptional level and mRNA is edited
what are the sections of DNA called that don’t code for amino acids
introns
what are the sections of DNA called that do code for amino acids
exons
what are mRNA strands called that contain introns and exons
primary mRNA transcripts
what happens during ‘splicing’ and where does it take place
introns are removed from the primary mRNA and exons are joined together to form mature mRNA
nucleus
some proteins have to be activated after they are made what is an example of this
cAMP (post-transcriptional level control)