cell trafficking

Question 1

in what direction do vesicles deliver content?

Answer 1

from donor to target compartments

Question 2

cargo molecules

Answer 2

brought to exit site, can be concentrated, collected in vesicle and vesicle will bud off and be free and work with target. lipid bilayers will fuse at target compartment. cargo can include membrane or soluble proteins

Question 3

vesicles

Answer 3

are characteristic sizes and very distinct shapes

Question 4

three types of protein coats

Answer 4

clathrin, COPI, COPII. these coats are somewhat restricted and function in particular trafficking relationships.

Question 5

clathrin

Answer 5

endocytosis/ later movements

Question 6

COPII

Answer 6

movement out of ER

Question 7

COPI

Answer 7

retrieval pathway overseen by COPI

Question 8

how are coats different?

Answer 8

each type of vesicle coat has a characteristic composition and ultrastructure. differences reflect what proteins are that are assembled into these structures.

Question 9

clathrin triskelion…

Answer 9

will form something that looks like a vesicle, but there is no membrane.

Question 10

assembly and disassembly of clathrin coat

Answer 10

at donor membrane, cargo receptor takes molecule, is attached to adaptor proteins, and multiple receptors bud off into a vesicel surrounded by a clathrin triskelion.

Question 11

how does clathrin triskelion bud off?

Answer 11

membrane bending and fission proteins.and then there is uncoating so that snare proteins can recognize each other.

Question 12

what components coordinate budding of coated vesicles?

Answer 12

coat proteins, small GTP-binding proteins

Question 13

COPII coated vesicle

Answer 13

takes from ER to cis golgi; forward transport out of the ER

Question 14

COPI

Answer 14

retrieve from cis golgi and bring back to ER

Question 15

cis golgi network

Answer 15

first part of golgi,