cell trafficking 2

Question 1

Rab GTPases and SNARES

Answer 1

there are more than 30 Rab GTPases and more than 20 SNAMREs that regulated specificity of membrane interactions and fusions

Question 2

RAB GTP ases

Answer 2

important in associations of membrane docking (involved in regulation) membrane snares cause interaction of fusing. there is als

Question 3

Rab effector

Answer 3

tethering protein in docking

Question 4

what is golgi

Answer 4

an ordered and compartmentalized organelle. the next step on teh way out . cis golgi. cis cisternak

Question 5

golgi

Answer 5

later FACE (trans Goli), cs FACE

Question 6

how is movement in and out of golgi complex?

Answer 6

direction, with secretory granules at the exit, (trans face)

Question 7

golgi complex organization

Answer 7

series of stacks

Question 8

make up of golgi

Answer 8

entry face, cis golgi network, sorting
phosphorylation of oligosaccharides on lysosomal proteins, in cis golgi network
cis cisterna, removal of Man
medial cisterna, removal of man, addn of glcmac
transcisterna, addion of gal, addition of nana
transgologi network, sulfation of tyrosines and carbs

Question 9

golgi stack

Answer 9

cis medial and trans cisterna

Question 10

what comes out of golgi?

Answer 10

lysosome, plasma membrane, secretory vesicle

Question 11

where does N-oligosaccharide processing start? continue?

Answer 11

ER. continues sequentially in Golgi cisterna

have to remove sugar residues before other sugars can be added.

Question 12

sorting in trans golgi network

Answer 12

mannose 6 phosphate recepotr send signal mediated diversion to lysosomes via endosomes.

2. signal mediated diversion to secretory vesicles (for regulated secretion)
3. constitutive secretory pathway

Question 13

exocytosis: constitutive and regulated secretion

Answer 13

from trans Golgi: either newly synthesized plasma membrane lipids or plasma membrane protein. newly syntehsized soluble proteins fro constitutive secretion, merges with membrane and make constitutive secretory pathway
regulated pathway: trans, secretory vesicle storing secretory proteins, regulated membrane fusion and regulated secretory pathway. responds to signal such as neurotransmitter or hormone.

Question 14

formation of secretory vesicle

Answer 14

golgi cisterna, trans golgi, clathrin coats immature secretory vesicle, but then as it matures, clathrin uncoats and is retrieved by trans golgi network

Question 15

how is insulin different in its processing?

Answer 15

concentrated and proteolytially processed from a proprotein after existing the trans golgi. proinsulin exits as proinsulin, but more dilute, less electron dense vesicles are stained with antibody.

Question 16

release of secretory granule contents by exocytosis

Answer 16

docks and fuses with plasma membrane and has access to outside of cell.

Question 17

what happens if cells are polarized and are receiving content?

Answer 17

proteins are sorted from the golgi complex to the apical or basolateral PM. for example, epithelial cells, nerve cells.

Question 18

direct sorting in the trans golgi network

Answer 18

polar sides separated by tight junctions. something sent to apical and something sent to basolateral.

Question 19

indirect sorting via early endosomes

Answer 19

interaction with endosomes

Question 20

golgi matrix

Answer 20

stabilizes structurral organization