Cell Migration Flashcards
1
Q
What does Mg+ do in GTPase binding?
A
Essential as it is strongly positive, and phosphate is strongly negative.
2
Q
What do switch regions bind to during GTPase binding>
A
Effectors
3
Q
What helps the catalysis of GTP hydrolysis?
A
- Position of water (catalytic glutamine positions the water)
- P-loop counteracts the negative charge of phosphate which destabilises the bonds
4
Q
What does GEF accelerate reactions by?
A
10-10^7 fold
5
Q
What does GAP accelerate reactions by?
A
2000-10^5 fold
6
Q
What is the most widely applicable GEF?
A
VAV. It will regulate alot of things
7
Q
What GEF only affects Rac?
A
Tiam1
8
Q
Outline actin mobility
A
- Cdc42 drives filopodium
- Rac drives forward movement
- Rhoa forms stress fibres to drive body forwards.
9
Q
What type of signals are Cdc42 and Rac?
A
Protrusive signals
10
Q
What type of signals are Rhoa in actin based motility?
A
Contractile signal
