Cell Level Systems Flashcards
Magnification
How much larger a displayed image is compared with the original object
Resolution
The ability to distinguish between two points
Why is staining used
- Some specimens are colourless
- It is useful to highlight different structures
- It increases contrast
What is the formula to calculate magnification from image size and actual size?
What is the formula to calculate magnification from image size and actual size?
(The I AM formula)
Image size = Actual Size x Magnification
Total magnification
Total magnification =
Eyepiece magnification x Objective lens
magnification
Light microscope
Place the specimen on a slide, cover it with a cover slip, illuminate with a lamp and view using the eyepiece
Parts of animal cell
Nucleus, cytoplasm, mitochondria, ribosomes and the cell membrane
Give 3 differences between prokaryotic and eukaryotic cells
- Prokaryotic cells have no nucleus
- Prokaryotic cells have plasmid loops of
DNA - Prokaryotic cells have no mitochondria
Chloroplasts
Site of photosynthesis
Vacuole
Supports the cell, contains cell sap
State 3 organelles only found in plant cells
- Cellulose cell wall
- Permanent vacuole
- Chloroplasts
What is cell wall in bacteria made up of
Peptidoglycan
Advantages of using TEM
Very high resolution
Very high magnification
Can see objects up to 2nm apart
Disadvantages of using TEM
Very expensive
Need to be serviced
Images in black and white
Advantages if using SEM
Faster than TEM
Can have a thicker sample
Less expensive than TEM