Cell Growth (p. 13-19)

Question 1

Very high, may be toxic to some organisms
-Cells require this for proper metabolic functions
-Is controlled by changing CO2 concentration in air supply and agitation speed

Answer 1

Dissolved Carbon Dioxide

Question 2

Affect the transport of certain nutrients in and out of the cell

Answer 2

Ionic Strength

Question 3

Single substrate, S, controls growth
-Is analogous to Michaelis-Menten enzyme kinetics and the Langmuir isotherm.

Answer 3

Monod equation

Question 4

Are not typically mechanistic, but are selected to fit the data

Answer 4

Modeling Growth Inhibition

Question 5

If a_________ is inhibiting cell growth of a batch culture, the substrate should be added in a _________.

Answer 5

Substrate; fed-batch mode

Question 6

If a ___________ were inhibiting cell growth of a batch culture, the product recovery will be expensive.

Answer 6

Product

Question 7

Used to represent changes in cell mass due to endogenous respiration and for changes in cell mass due to cell death and lysis.

Answer 7

Kd (death rate constant)

Question 8

Catabolism of cellular reserves for continued maintenance and energy

Answer 8

Endogenous respiration

Question 9

Energy expenditures for repair, transport, mobility

Answer 9

Maintenance

Question 10

Consumption of storage polymers for energy

Answer 10

Endogenous metabolism

Question 11

The quantitative relationship between two or more substances especially in processes involving physical or chemical change

Answer 11

Stoichiometry

Question 12

If cells have a characteristic molecular composition (i.e.; CH₂ON) then ______ can be determined through stoichiometry. In practice, these will be estimates.

Answer 12

Yield coefficients

Question 13

continuous culture instrumentation: (6)

Answer 13

•pH probe and controller
•DO probe and controller
•Antifoam probe and controller
•Level probe and controller
•Nutrient addition pump
•Agitation rate controller