Cell Bio VI Flashcards
What is different in the Tau in Alzheimer’s
hyperphosphorylated
Phallacidin labels what
actin microfilaments (green)
MitoTracker red labels what
mitochondria (red)
DAPI stains what
nuclear DNA (blue)
Cytochalasins
inhibit actin polymerase
Cytokeratins are located where
epithelial cells
Desmin is located where
muscle (smooth and striated)
Glial gibrillary acid protein (GFAP) is located where
astrocytic glial cells
Neurofilament protein is located where
neurons
Nuclear lamin is located where
nucleus of all cells
Vimentin is located wehre
many mesodermal tissues
Neurofilaments
confer great tensile strength to axons. Form extensive cross bridges
Glial intermediate filaments
infrequent cross bridges
Mutations in keratin 5 or 14 gene do what
prevent keratin from assembling into strong networks. Epidermis becomes fragile/easily damages
Rosenthal’s fibers
Aggregates of GFAP and other proteins. Characteristic of pilocytic astrocytoma
Centrosome
lies next to the nucleus. Made of 9 triplet sets of microtubules
Well differented tumor
more likely to be benign than poorly differentiated tumor
PML bodies
involved in DNA repair and apoptosis
Cajal bodies
involved in splicing
Nuclear envelope
- 2 lipid bilayer
- has outer membrane, perinucleur space, inner membrane, nuclear lamina, then chromatin
Nuclear pore
Small materials can pass through easily
Lamina
made of intermediate filament
Heterochromatin
tightly coiled. Darker appearance
Euchromatin
Not tightly coiled. Lighter appearance
Orphan Annie Eye Nuclei
Hallmark of papillary thyroid cancer. Cells look empty.
